www.nature.com/scientificreports OPEN Optimisation of robust singleplex and multiplex droplet digital PCR assays for high confidence Received: 12 November 2018 Accepted: 12 August 2019 Published: xx xx xxxx mutation detection in circulating tumour DNA Vicky Rowlands 1, Andrzej J. ...
Here we describe how we have developed and systematically validated robust exploratory singleplex and multiplex ddPCR assays for the detection and monitoring of tumour-derived point mutations in human plasma using the Bio-Rad QX200 platform with probe-based detection. To minimise the volume of clinica...
Performance differed substantially among assays and only one assay was found useful for telomere length estimation in humpback whales. The most notable factors causing these inter-assay differences were primer design and choice of using singleplex or multiplex assays. Inferred amplification efficiencies dif...
The QIAstat-Dx Meningitis/Encephalitis (QIA/ME) is a molecular syndromic panel that enables the simultaneous detection of multiple pathogens and provides the visualization of cycle threshold (Ct) values, offering rapid results for prompt clinical management. This study retrospectively tested, with the ...
(DNA-template gradients vs. # PCR cycles), (C) to co-amplify conserved regions of the prokaryotic 16S rRNA using “universal primers” to switch from OD/AU to “normalized units” (semiquantification), (D) to migrate this “single plex” endpoint 2S-PCR protocol to a multiplex assay and...