While single-cell analyses yielded insights into T cell behaviors, in most cases, their antigen specificities are largely unknown, which hinders the understanding of tumor-reactive T cell responses, especially
Although lung cancer risk among smokers is dependent on smoking dose, it remains unknown if this increased risk reflects an increased rate of somatic mutation accumulation in normal lung cells. Here, we applied single-cell whole-genome sequencing of prox
而新一代测序技术的出现,如单细胞RNA测序(single-cell RNA sequencing, scRNA-seq)、DNA测序(single-cell DNA sequencing, scDNA-seq)和蛋白质测序(single-cell proteomics, scPro-seq)等,使得从单个细胞中获取各种组学信息成为可能。 在单细胞多组学研究中,单细胞RNA测序是最常用的方法之一。它可以对单个细胞的...
634471SMART-Seq® Single Cell Kit48 Rxns* The SMART-Seq Single Cell Kit is designed to generate high-quality, full-length cDNA directly from single cells. It has been validated with 2 pg of total RNA input and with single cells known to have low RNA content (e.g.,...
To systematically analyze the cell composition and transcriptome of primary human endometrial stromal cells (HESCs) and transformed human endometrial stromal cells (THESCs). Design The primary HESCs from 3 different donors and 1 immortalized THESC were collected from the human endometrium at the midsec...
Scanpy is a scalable toolkit for analyzing single-cell gene expression data built jointly withanndata. It includes preprocessing, visualization, clustering, trajectory inference and differential expression testing. The Python-based implementation efficiently deals with datasets of more than one million cells...
single-cell RNA sequencing immune cells developmental dynamics human fetal skin metabolic reprogramming macrohages innate lymphoid cells Introduction The skin, which is the outermost organ of the body, blocks pathogen entry and protects against damage from the environment. Mammalian skin has three anatomic...
Single-cell Lung Cancer Atlas with 1.2M cells. Contribute to icbi-lab/luca development by creating an account on GitHub.
We assigned a cell cycle phase (G1, S or G2/M) to each single cell using the ‘cyclone’ function in the Scran R package (version 1.28.1). Total RNA sequencing data processing Total RNA of THP-1 cells which were treated by U73122 (5µM) or DMSO (0.1%) for 24 h (n =...
The living microarray: a high- throughput platform for measuring transcription dynamics in single cells. BMC Genomics. 2011:12. [PubMed: 21211037]Rajan, S.; Djambazian, H.; Dang, H.C.; Sladek, R.; Hudson, T.J. The living microarray: A high-throughput platform for measuring transcription...