而新一代测序技术的出现,如单细胞RNA测序(single-cell RNA sequencing, scRNA-seq)、DNA测序(single-cell DNA sequencing, scDNA-seq)和蛋白质测序(single-cell proteomics, scPro-seq)等,使得从单个细胞中获取各种组学信息成为可能。 在单细胞多组学研究中,单细胞RNA测序是最常用的方法之一。它可以对单个细胞的...
Single cell expression profiling (single cell RNA-seq, scRNA-seq) uses microfluidic devices to isolate single cells and generate barcoded cDNA libraries. The libraries are then sequenced by next-generation sequencing36,37. The sequence reads can then be decoded and attributed back to specific genes...
所有T细胞的TCR总和称为TCR库(TCR repertoire),每个人的TCR库是高度多样化的,这种多样化有利于识别更多的外源抗原,但同时也对利用传统方法鉴定TCR库构成了挑战,因此,亟需一种快捷高效精准的方法来研究TCR库。 高通量测序技术的出现使研究人员第一次能够大批量对TCR序列进行平行分析,本文总结了将高通量测序技术和单细胞...
Single cellSingle-cell Hi-CSci-Hi-CThree-dimensional (3D) genome architectureThe highly dynamic nature of chromosome conformation and three-dimensional (3D) genome organization leads to cell-to-cell variability in chromatin interactions within a cell population, even if the cells of the population ...
Single-cell analysis in Python. Scales to >100M cells. scanpy.readthedocs.io Topics python data-science machine-learning bioinformatics transcriptomics visualize-data scanpy anndata scverse Resources Readme License BSD-3-Clause license Code of conduct Code of conduct Activity Custom properties...
Naïve CD4+ T cells coordinate the immune response by acquiring an effector phenotype in response to cytokines. However, the cytokine responses in memory T cells remain largely understudied. Here we use quantitative proteomics, bulk RNA-seq, and single
634471SMART-Seq® Single Cell Kit48 Rxns* The SMART-Seq Single Cell Kit is designed to generate high-quality, full-length cDNA directly from single cells. It has been validated with 2 pg of total RNA input and with single cells known to have low RNA content (e.g.,...
If you find cellSNP (the predecessor ofcellsnp-lite) is useful for your research, please cite: Xianjie Huang, Yuanhua Huang, Cellsnp-lite: an efficient tool for genotyping single cells, Bioinformatics, 2021;, btab358,https://doi.org/10.1093/bioinformatics/btab358 ...
Single-cell RNA sequencing reveals the dynamics and heterogeneity of lymph node immune cells during acute and chronic viral infections 单细胞RNA测序揭示急性和慢性病毒感染期间淋巴结免疫细胞的动态和异质性 相关领域 免疫系统 免疫学 生物 慢性感染 CD8型 淋巴结 细胞毒性T细胞 T细胞受体 T细胞 病毒学 遗传...
在这里,我们提出,可以通过区分常见单细胞RNA测序方案中Unspliced mRNAs和Spliced mRNAs来直接估计RNA velocity(基因表达状态的时间导数)。 RNA velocity是一个高维向量,可以在几小时的时间尺度上预测单个细胞的未来状态。 我们在神经脊谱系中验证其准确性,展示其在多个已公开数据集和技术平台上的应用,揭示发育中小鼠海马...