cellSNPPublic Pileup biallelic SNPs from single-cell and bulk RNA-seq data single-cell-genetics/cellSNP’s past year of commit activity Python77Apache-2.011110UpdatedJun 9, 2022 People This organization has no public members. You must be a member to see who’s a part of this organization. ...
单细胞测序技术是当下的一个热点,发了不少大文章,逐渐成为 CNS 的标配了。目前,单细胞测序相对来讲还是属于比较昂贵的技术,没钱做不起怎么办? 不用担心,有很多数据库可以用!比如: HCPortalen(网址:http://single-cell.clst.riken.jp),一个人类与小鼠的单细胞测序数据库; scRNASeqDB(网址:https://bioinfo....
# 1.构建对象 min.cells = 0 # min.cells 某一个基因至少在多少个基因中表达 min.features = 0 # min.features 某个细胞至少表达多少个基因 sce = CreateSeuratObject(counts = raw.data,metadata = metadata,min.cells =min.cells,min.features =min.features) sce = AddMetaData(object = sce,metadata ...
cells=epi.cells), slot='counts')) # 间质细胞 cells.use = row.names(sce@meta.data)[which(phe$immune_annotation=='stromal')] sce =subset(sce, cells=cells.use) load(file = 'phe-of-subtypes-stromal.Rdata') fib.cells = row.names(sce@meta.data)[phe$singleR=='Fibroblasts'] endo.cells...
Mass spectrometry (MS), the most unbiased technique for single-cell proteomics, facilitates the identification and quantification of thousands of proteins, proteoforms, and post-translational modifications (PTMs) in individual cells. A successful single-cell proteomics workflow hinges on through...
The development of single-cell and spatial transcriptomics methods was instrumental in the conception of the Human Cell Atlas initiative, which aims to generate an integrated map of all cells across the human body. These technology advances are bringing increasing depth and resolution to maps of huma...
The labels a1,an,...,d1,dn denote individual single cells of cell type a,a,…,d,d, respectively. b–d, Technical assessment of CytoSPACE. b, Framework for evaluating CytoSPACE using simulated ST datasets with fully defined single-cell composition and spot resolution (Methods). c, Heat ...
作者没有直接进行单细胞测序,而是先进行了普通的转录组测序。从酶分解消化标记的骨髓中分离出CD45low TER119lowtdTomato+ cells细胞并进行转录组测序(bulkmRNA sequencing), 结果证明存在不同的转录类群。然后才进行单细胞转录组测序。 选择VE-Cad+ , LEPR+ 与COL2.3+ 阳性的细胞进行了单细胞测序,来研究骨髓龛细胞...
Dispense single cells with high occupancy and viability. Precisely dispense picoliter through microliter volumes of reagents all on the same platform. C1 Dispensehead Cassette Dispense up to 400 single cells and 20 microliter fill volume. T1 Dispensehead Cassette Dispense with minimum dispense volume...
Our single-cell transcriptomic dataset delineates the inter-tumor heterogeneity of human ICCs, underlining the importance of intercellular crosstalk between ICC cells and vCAFs, and revealing potential therapeutic targets. Lay summary Intrahepatic cholangiocarcinoma is an aggressive and chemoresistant malignancy...