single cell Raman microscopy3D cell scaffolds3D mappingStudying cells in a threeヾimensional (3D) environment has great potential in understanding cell behaviours such as morphology, proliferation, differentiation, and migration. Microengineered 3D cell scaffolds with precise defined geometries have offered...
RESEARCH ARTICL ESingle‐cell Raman microscopy of microengineeredcell scaffoldsSara J. Baldock 1 | Abdullah C.S. Talari 1 | Rachael Smith 1 | Karen L. Wright 2 |Lorna Ashton 11 Department of Chemistry, LancasterUniversity, Lancaster, UK2 Faculty Health and Medicine, Division ofBiomedical and L...
To address these limitations, we envision a ‘screen-first, culture-second’ strategy instead, called single-cell Raman-activated Cell Sorting and Culturing (scRACS-Culture), where, at resolution of precisely one cell and directly from the environmental sample, cells of targeted function are screene...
The manipulation and analysis of single cells have enabled various applications in the field of medical and life sciences. Microfluidic single-cell manipulation uses designed flows in a microchannel network and plays an important role in precise and high-throughput manipulation of single cells. Unlike ...
Kobayashi-Kirschvink, K. J. et al. Prediction of single-cell RNA expression profiles in live cells by Raman microscopy with Raman2RNA.Nat. Biotechnol.https://doi.org/10.1038/s41587-023-02082-2(2024). ArticleGoogle Scholar Vayer, T., Chapel, L., Flamary, R., Tavenard, R. & Courty,...
We utilized a commercial confocal Raman microspectroscope (LabRAM HR Evolution; HORIBA Scientific) based on an inverted microscope (Eclipse Ti; Nikon) with two cameras: an sCMOS camera (ORCA-Flash 4.0; Hamamatsu Photonics; field of view of 221.867 μm × 221.867 μm; camera #1)...
8.Single-Cell Raman Spectral Analysis of Oxygenated and Deoxygenated Thalassemia Erythrocytes单细胞拉曼光谱分析地中海贫血红细胞的氧合态和去氧态 9.Clinical Features of Infectious Mononucleosis in Adulthood: An Analysis of 7 Cases;7例成人传染性单核细胞增多症的临床分析 10.Analysis of Clinical Data of ...
Monitoring the healing of combat wounds using Raman spectroscopic mapping. Wound Repair Regen. 2010;18:409–16. Article PubMed Google Scholar Efremova M, Vento-Tormo M, Teichmann SA, Vento-Tormo R. CellPhoneDB: inferring cell-cell communication from combined expression of multi-subunit ligand-...
1.Researches on the technology of single cell PCR;单细胞PCR技术的研究进展 2.This paper discusses the principle of Raman spectrum and the development of it s use in tissue entity,histological sections,serum and single cell of malignant tu- mor patients.本文综述了拉曼光谱原理及其在恶性肿瘤患者的组...
(2021). Optofluidic Raman-activated cell sorting for targeted genome retrieval or cultivation of microbial cells with specific functions. Nat Protoc 16, 634–676. Article CAS PubMed Google Scholar Leighton, J., Hu, M., Sci, E., Meric-Bernstam, F., and Navin, N.E. (2023). ...