single cell Raman microscopy3D cell scaffolds3D mappingStudying cells in a threeヾimensional (3D) environment has great potential in understanding cell behaviours such as morphology, proliferation, differentiation, and migration. Microengineered 3D cell scaffolds with precise defined geometries have offered...
To address these limitations, we envision a ‘screen-first, culture-second’ strategy instead, called single-cell Raman-activated Cell Sorting and Culturing (scRACS-Culture), where, at resolution of precisely one cell and directly from the environmental sample, cells of targeted function are screene...
In vivo lipidomics using single-cell Raman spectroscopy. H Wu,JV Volponi,AE Oliver,AN Parikh,BA Simmons,S Singh. Proceedings of the National Academy of Sciences of the United States of America . 2011Wu H, Volponi JV, OliverAE, et al. In vivo lipidomics using single-cell Raman ...
non-invasiveway.Ramanmicroscopyappearstobeasuitablemethodfor studyingpopulationdistributionsinbacterialcultures.©2000ElsevierScienceB.V.Allrightsreserved. Keywords:Singlecellanalysis;Bacteria;Ramanmicroscopy;Chemicalcomposition;Heterogeneity 1.Introductionrenewableresourcesandagriculturalwastesinto basicchemicalsandliquid...
8.Single-Cell Raman Spectral Analysis of Oxygenated and Deoxygenated Thalassemia Erythrocytes单细胞拉曼光谱分析地中海贫血红细胞的氧合态和去氧态 9.Clinical Features of Infectious Mononucleosis in Adulthood: An Analysis of 7 Cases;7例成人传染性单核细胞增多症的临床分析 10.Analysis of Clinical Data of ...
To conclude, Raman spectroscopy allows label-free, continuous monitoring of cell growth, which may facilitate more accurate estimates of the growth states of lactic acid bacterial populations during fermented batch culture in industry. 展开 关键词: Lactobacillus caseiZhang Growth phases Single-cell Raman...
We employed a function-oriented single-cell Raman approach to identify, quantify and sequence active phosphorus solubilizing bacteria from complex soil matrices. The in situ behaviour of phosphorus solubilizing bacteria across diverse soil and fertilization regimes, as well as the key taxa driving active...
Under these conditions expected changes occurred after a few hours and were revealed by the reduction of cytochrome c, lipid, nucleic acid and protein Raman signals: this ensured the possibility to analyse U251 cell line as grown on Si substrate, and to monitor the response of single cells to...
摘要: Raman-activated cell sorting (RACS) systems are significant protocol for characterizing phenotypes and genotypes of individual bacteria.关键词: Biomarkers Machine learning Raman-activated cell sorting Single cell Stable isotope probing Surface-enhanced Raman scattering DOI: 10.1007/s00253-020-11081-...
We utilized a commercial confocal Raman microspectroscope (LabRAM HR Evolution; HORIBA Scientific) based on an inverted microscope (Eclipse Ti; Nikon) with two cameras: an sCMOS camera (ORCA-Flash 4.0; Hamamatsu Photonics; field of view of 221.867 μm × 221.867 μm; camera #1)...