In preferred embodiments, two spectra are subtracted from each other to give a difference spectrum and each channel is analyzed independently to characterize the trapped cell. Alternatively, the Raman spectrum can be subjected to Principal Component Analysis (PCA) in order to characterize the trapped ...
To address these limitations, we envision a ‘screen-first, culture-second’ strategy instead, called single-cell Raman-activated Cell Sorting and Culturing (scRACS-Culture), where, at resolution of precisely one cell and directly from the environmental sample, cells of targeted function are screene...
The Raman spectrum of a cell is a biochemical 'fingerprint', containing molecular-level information about all biopolymers contained within the cell. The high information content of Raman spectra can be used to characterize the distribution of multiple cellular components, and to study the dynamics of...
A supervised classification model specified apoptotic and necrotic cell death based on single cell Raman spectra. To conclude, Raman spectroscopy allows a non-invasive, continuous monitoring of cell death, which may help shedding new light on complex pathophysiological or drug-induced cell death ...
After anaerobically incubating the human faeces with heavy water (D2O), D2O with specific substrates (glucose, tyrosine, tryptophan and oleic acid) and deuterated glucose, the C–D band in single‐cell Raman spectra appeared in some bacteria in faeces, due to the Raman shift from the C–H ...
In order to reveal the heterogeneous cell response, single cell analysis is an essential requirement. In this study, optical microscopy and Raman microspectroscopy were used to follow the stress response of U251 single cells adherent on a silicon substrate. Cultured cells on silicon substrate were ...
For heterogeneous samples like single spores or yeast cells a mean spectrum from up to ten different positions is required to describe the complete cell. Using micro-Raman spectra of single bacterial cells and average spectra of yeast cells it is possible to create a database and identify ...
A single-crystal X-ray diffraction study shows that La2(SO403 · 8H2O crystallizes in the monoclinic space group Pn (Z = 2) with cell dimensions a = 6.881(2) Å, b = 17.376(4) Å, c = 6.923(2) Å, and β = 92.34 (2)°. The structure was refined to a final R = ...
A challenge, particularly for crystals with many different sites and/or several asymmetric units in the unit cell, is to establish an unambiguous assignment of resonances for different rotation angles in the so-called rotation plots. The simplest but time-consuming solution is to use small time st...
In order to further remove the interference of the fluorescence and stray light from the trapped cell, we employed a shifted excitation Raman difference technique with slightly tuned laser frequencies. With this system, high-quality Raman spectra were obtained from single optically trapped biological ...