Fig. 1: Single-cell measurements of DMSP degradation pathway expression. a,bPlasmids transformed intoR. pomeroyiDSS-3 contain three components:dmdAreporter (222 bp promoter region);dddWreporter (500 bp promoter region); and constitutiveyfpexpression (strong, synthetic promoter PA1/04/03). YFP ...
Existing single-cell atlases of zebrafish development document the emergence of diverse cell types from 3.3 h (pregastrulation) to 5 days (late organogenesis) post-fertilization, in addition to a few selected mutants at a single timepoint7,8,9. While these datasets resolved diverse cellular...
et al. Joint single-cell measurements of nuclear proteins and RNA in vivo. Nat Methods 18, 1204–1212 (2021). https://doi.org/10.1038/s41592-021-01278-1 Download citation Received11 January 2021 Accepted19 August 2021 Published04 October 2021 Issue DateOctober 2021 DOIhttps://doi.org/...
Changes in the Heterogeneity of MSC Subpopulations During Replicative Senescence as Seen from Single-Cell Speed Measurementsdoi:10.1134/S106816202408020Xreplicative senescencecell motilitymesenchymal stem cellsheterogeneitydistribution modalitysingle-cell motility analysis...
Reduced number of measurements needed to draw conclusions High confidence with deep proteome coverage and data completeness 100s-1000s of proteins from a single cell Wide dynamic range to discover differentially expressed low-abundance proteins of biological significance Orbitrap based DIA singl...
we must show that heteroplasmic mtDNA mutations (1) can be reliably detected in single cells, (2) are propagated in daughter cells, (3) can be used to accurately determine clonal relationships, (4) can be combined with cell state measurements to learn meaningful biology, and (5) can be ...
Here, we generalize t-SNE and UMAP to the joint visualization of multimodal single-cell measurements. While t-SNE and UMAP seek a low-dimensional embedding of cells that preserves similarities in the original (e.g., gene expression) space as well as possible, we propose j-SNE and j-UMAP ...
Cell Picking Selective Single Cell Isolation Single Cell Sorting Laser Microdissection 1 2 3 4 5 6 Molecular Machines & Industries (MMI): Your Partner in Microdissection – Micromanipulation – Imaging We at Molecular Machines & Industries (MMI) understand that your lab equipment needs to meet the...
在这篇文章中,作者首先基于 the errors and ambiguities that can arise in inferring dynamics directly from single- cell state measurements 引出谱系示踪,接着argued that the integration of lineage- barcoding data can improve state manifold representations by facilitating a faithful reconstruction of dynamics...
Two common measurements are adopted as test statistics to characterize the performance of cell clustering, such as adjusted rand index (ARI) and normalized mutual information (NMI) (detailed definition in Additional file 1: Supplementary Note S4). The higher the value of the two metrics, the ...