Fig. 1: Single-cell measurements of DMSP degradation pathway expression. a,bPlasmids transformed intoR. pomeroyiDSS-3 contain three components:dmdAreporter (222 bp promoter region);dddWreporter (500 bp promoter region); and constitutiveyfpexpression (strong, synthetic promoter PA1/04/03). YFP ...
et al. Joint single-cell measurements of nuclear proteins and RNA in vivo. Nat Methods 18, 1204–1212 (2021). https://doi.org/10.1038/s41592-021-01278-1 Download citation Received11 January 2021 Accepted19 August 2021 Published04 October 2021 Issue DateOctober 2021 DOIhttps://doi.org/...
Here, we present single-cell measurements of the expression of these two pathways using engineered fluorescent reporter strains of Ruegeria pomeroyi DSS-3, and find that external DMSP concentration dictates the relative expression of the two pathways. DMSP induces an upregulation of both pathways, ...
在这篇文章中,作者首先基于 the errors and ambiguities that can arise in inferring dynamics directly from single- cell state measurements 引出谱系示踪,接着argued that the integration of lineage- barcoding data can improve state manifold representations by facilitating a faithful reconstruction of dynamics...
The purpose of this paper is to describe the development of a tool that will allow more accurate measurements of the phase change temperature for a single cell (100 μm diameter) as it freezes. The resulting data will be used to improve the fundamental cryopreservation model. A proof-of-...
Here, we generalize t-SNE and UMAP to the joint visualization of multimodal single-cell measurements. While t-SNE and UMAP seek a low-dimensional embedding of cells that preserves similarities in the original (e.g., gene expression) space as well as possible, we propose j-SNE and j-UMAP ...
SCIMITAR provides a variety of tools to analyze trajectory maps of single-cell measurements. With SCIMITAR you can: Obtain coarse-grain, (metastable) state and transition representations of your data. This is useful when you want to get a broad sense of how your data is connected. Infer full...
Single-cell proteomics workflows have considerably improved in sensitivity and reproducibility to characterize as-yet unknown biological phenomena. With the emergence of multiplexed single-cell proteomics, studies increasingly present single-cell measurements in conjunction with an abundant congruent carrier to ...
In our previous publication2, we selected cell lineages that contained measurements of full 70 generations. Also, we considered cells were filamentous when (1) initial cell length was larger thanL¯+2σL(L¯andσLare the average and s.d. of the cell size distribution, respectively) or (...
Although anecdotal evidence of the value of capturing heterogeneity abounds, it has remained puzzling that so-called average profiling, the practice of feature-averaging all single-cell measurements together using measures-of-center (mean or median), has remained the top-ranked approach in the field...