Single-cell measurements with mass spectrometry. New frontiers in ultrasensitive bioanalysis: advanced analytical chemistry applications in nanobiotechnology, single molecule detection, and single cell analysis, 269, (2007).Monroe, E, Jurchen, J, Rubakhin, S, Sweedler, J Single-cell measurements with ...
Single-cell Raman measurements were performed (1.5-s exposure time; 150-μm pinhole size) by manually moving thexy-stage to align the Raman laser (532 nm, 400 mW power), as well as to focus it (confirmed via inspection with camera #2), onto each cell. For each experimental conditi...
et al. Joint single-cell measurements of nuclear proteins and RNA in vivo. Nat Methods 18, 1204–1212 (2021). https://doi.org/10.1038/s41592-021-01278-1 Download citation Received11 January 2021 Accepted19 August 2021 Published04 October 2021 Issue DateOctober 2021 DOIhttps://doi.org/...
Here, we develop a strategy to “anchor” diverse datasets together, enabling us to integrate single-cell measurements not only across scRNA-seq technologies, but also across different modalities. After demonstrating improvement over existing methods for integrating scRNA-seq data, we anchor scRNA-seq ...
Here, we present single-cell measurements of the expression of these two pathways using engineered fluorescent reporter strains of Ruegeria pomeroyi DSS-3, and find that external DMSP concentration dictates the relative expression of the two pathways. DMSP induces an upregulation of both pathways, ...
Here, we develop a strategy to “anchor” diverse datasets together, enabling us to integrate single-cell measurements not only across scRNA-seq technologies, but also across different modalities. After demonstrating improvement over existing methods for integrating scRNA-seq data, we anchor scRNA-seq...
The purpose of this paper is to describe the development of a tool that will allow more accurate measurements of the phase change temperature for a single cell (100 μm diameter) as it freezes. The resulting data will be used to improve the fundamental cryopreservation model. A proof-of-...
“anchor” diverse datasets together, enabling us to integrate single-cell measurements not only across scRNA-seq technologies, but also across different modalities.After demonstrating improvement over existing methods for integrating scRNA-seq data, we anchor scRNA-seq experiments with scATAC-seq to ...
technology. Here we present data from single-cell measurements ofEscherichia coligrowth over 70 generations obtained for three different growth conditions. The data were recorded every minute, and contain time course data of cell length and fluorescent intensity of constitutively expressed yellow ...
The scSpaMet registered single-cell measurements in bi-modality and enabled accurate identification of various cell types with their corresponding metabolomic profiles. In summary, scSpaMet allows high-resolution joint protein and metabolite profiling at the single-cell level in the same tissue. Using ...