or unwound to provideDNA polymerases, strandtransferases,primases,RNApolymerases, and other proteins with access tosingle-stranded DNA(ssDNA).Enzymesable to catalyze the unwinding of double-stranded DNA (dsDNA) were first discovered over three decades ago[1,2]. Referred to as DNA helicases, ...
RNA is always single-stranded and is often referred to as single-stranded ribonucleic acid. This can be seen in Figure 1 below. However, DNA can be either double-stranded or a single strand of DNA (ssDNA).Figure 1. The comparison between single-stranded RNA and double-stranded DNA. Image...
The invention discloses the use of single-stranded RNA toeholds of different lengths to promote the re-association of various RNA-DNA hybrids, which results in activation of multiple split functionalities inside human cells. Previously designed RNA/DNA nanoparticles employed single-stranded DNA toeholds...
Antisense long noncoding RNAs (lncRNAs), which often overlap with the transcripts encoded from the sense strand, constitute the largest sub-class of lncRNAs1,2. They have been shown to regulate the expression of overlapping sense transcripts positively or negatively. Diverse antisense lncRNA-regulate...
SOS induction in Escherichia coli by single-stranded DNA of mutant filamentous phage: monitoring by cleavage of LexA repressor. Infection of Escherichia coli in the presence of chloramphenicol with mutant filamentous phage that are defective in the initiation of minus-strand DNA syn... N Higashitani...
Synthetic gene networks in mammalian cells are currently limited to either protein-based transcription factors or RNA-based regulators. Here, we demonstrate a regulatory approach based on circular single-stranded DNA (Css DNA), which can be used as an ef
42.1). In the absence of a nascent DNA chain, how does DNA polymerase get started? This problem is solved on the lagging strand by a DNA-dependent RNA polymerase called a primase, which, like other RNA polymerases, can initiate synthesis de novo without the need for a 3′OH group. In ...
The reverse transcriptase is then able to convert from mRNA to the DNA of the TSO and synthesize a cDNA strand using the helper oligonucleotide as a template. The full-length cDNA synthesized by this method contains the complete 5′ end of mRNA concurrently reduces the 3´ bias of ...
Various internal and external stresses induce many types of DNA damage such as chemical change of bases, single-strand break (SSB) and double-strand break (DSB). On DNA damage, the DNA damage response (DDR) signalling is activated to repair damaged DNA. Recruitment and autophosphorylation of at...
W. Transcription bypass or blockage at single-strand breaks on the DNA template strand: effect of different 3′ and 5′ flanking groups on the T7 RNA polymerase elongation complex. Biochemistry 33, 14926–14934 (1994). Article CAS PubMed Google Scholar Kathe, S. D., Shen, G. P. & ...