Single-Particle Tracking Palm of Nav1.6 in Hippocampal Neurons Demonstrates Unique Subcellular Diffusion LandscapesNitrogenaseIron-molybdenum cofactorFerredoxinEPRNifBBiosynthesis of metal clusters for the nitrogenase component proteins NifH and NifDK involves electron donation events. Yet, electron donors ...
...-Schwartz 的研究 团队将PALM 技术和单颗粒示踪技术(single-particle tracking)结合,成功地 观测到活体细胞胞膜蛋白的运动 …www.docin.com|基于1 个网页 2. 单粒子示踪技术 ...这种看不见的“流动状态”进行观察,这三种方法分别是单粒子示踪技术(single-particle tracking)、相关光谱学技术(correlati…www.lif...
Single-molecule and single-particle tracking have shown that the actin homologue MreB moves in a circumferential pattern around the bacterial cell, driven by cell wall synthesis. Other cytoskeletal protein structures, such as the FtsZ ring and PopZ nanodomains, have been characterized at subdiffraction...
Measuring absolute number concentrations of nanoparticles using single-particle tracking Single-particle tracking (SPT) microscopy is increasingly used to characterize nanoparticulate systems. We introduce a concept for estimation of particle n... M Röding,H Deschout,K Braeckmans,... - 《Physical Re...
Single-molecule counting with PALM Several important cellular functions involve low-copy number proteins that are not detectable by conventional measurement techniques (Ghaemmaghami et al.2003). Also, studies dealing with the stochastic nature of gene expression and its importance in biology (Elowitz et...
Super-resolution imaging and single-particle tracking require cells to be immobile as any movement reduces the resolution of the measurements. Here, we present a method based on APTES-glutaraldehyde coating of glass surfaces to immobilize cells without c
We combined photoactivated localization microscopy (PALM) with live-cell single-particle tracking to create a new method termed sptPALM. We created spatial... S Manley,JM Gillette,GH Patterson,... - 《Nature Methods》 被引量: 1053发表: 2008年 Advances in single-molecule fluorescence methods for...
Methods for imaging and tracking single molecules conjugated with fluorescent probes, called single-molecule tracking (SMT), are now providing researchers with the unprecedented ability to directly observe molecular behaviors and interactions in living c
Nanoscale curvature was created by bursting GUVs over polystyrene NPs of 50 and 70 nm radius and measured with super-resolution single-molecule fluorescence localization microscopy and single particle tracking (SPT). Results were compared to Monte Carlo simulations that reproduce the experimental data...
Examples of simulations that can be performed include PALM, dSTORM, sptPALM, PAINT, in 2D or 3D.Multicolor experiments can be simulated with unlimited number of colors.Complex laser excitation schemes can be simulated with unlimited number of lasers....