Definition In single‐particle tracking (SPT) high‐speed and high‐sensitivity video microscopy is employed to trace the movements of single particles – in this context usually single lipid, protein or nucleic acid molecules or single molecular complexes composed of them – on membranes (artificial...
Single-particle tracking photoactivated localization microscopy of membrane proteins in living plant tissues. Nat. Protoc. 16, 1600–1628 (2021). Article Google Scholar Mishra, K. et al. Genetically encoded photo-switchable molecular sensors for optoacoustic and super-resolution imaging. Nat. ...
Single-particle tracking photoactivated localization microscopy of membrane proteins in living plant tissues. Nat. Protoc. 16, 1600–1628 (2021). Article Google Scholar Mishra, K. et al. Genetically encoded photo-switchable molecular sensors for optoacoustic and super-resolution imaging. Nat. ...
Single-particle tracking photoactivation light microscopy (sptPALM) overcomes the concentration limit for SPT by photoconverting a small subset of ‘dark’ molecules at a time6. While this method achieves high frame rates, it only yields very short trajectories (typically <20 frames)7 because of ...
Analysis of the trajectories of small particles at high spatial and temporal resolution using video enhanced contrast microscopy provides a powerful approach to characterizing the mechanisms of particle motion in living cells and in other systems. We present here the theoretical basis for the analysis ...
Single particle trackingLive-cell microscopyIn recent years, microRNAs (miRNAs) have emerged as important gene-regulators and showed great potential in gene therapy. However, the knowledge of their function in vivo is still lacking. In this work, the exogenous miR-21 transfect...
Aim:The aim of this study was to evaluate the effect of the surface functionalization of model nanoparticles on their mobility in bacterial biofilms and cystic fibrosis sputum.Materials & methods:With single-particle tracking microscopy, the mobility of 0.1- and 0.2-m fluorescent polyethylene glycol ...
The methods include single particle tracking of quantum-dot labeled AQP4, and total internal reflection fluorescence microscopy of green fluorescent protein (... JM Crane,M Tajima,AS Verkman - 《Neuroscience》 被引量: 55发表: 2010年 Imaging Live Cells at the Nanometer-Scale with Single-Molecule ...
Label-Free, Ultrahigh-Speed, Direct Imaging and Tracking of Bionanoparticles in Live Cells by Using Coherent Brightfield Microscopy Chapter © 2019 Introduction Fluorescence single-particle tracking (SPT) is a fundamental tool for investigating the functions of individual particles in biological environm...
bodies in the nucleus (left panel). Representative maximum intensity projection of nuclear RING1B-HT-JF549signal acquired by spinning disk microscopy (n = 63 cells). The yellow arrowhead indicates a single Polycomb body. Scale bar = 5 μm (right panel).iFRAP recovery curves for ...