Single-particle tracking (SPT) is a powerful method for analysing these behaviours directly and in live cells. However, SPT is confounded by multiple sources of experimental noise and biases. Interpreting tracks in terms of quantitative models thus remains a challenging task. We start this Primer ...
Single-particle tracking (SPT) is a powerful method for analysing these behaviours directly and in live cells. However, SPT is confounded by multiple sources of experimental noise and biases. Interpreting tracks in terms of quantitative models thus remains a challenging task. We start this Primer ...
Fig. 1: Deep learning-driven adaptive optics for single-molecule localization microscopy. Upon the acquisition of camera frames, detected single-molecule emission patterns from stochastic lateral and axial positions are isolated and sent to a trained DNN. The network outputs a vector of mirror deforma...
Classification of diffusion modes in single-particle tracking data: Feature-based versus deep-learning approach 来自 arXiv.org 喜欢 0 阅读量: 54 作者:P Kowalek,H Loch-Olszewska,J Szwabiński 摘要: Single-particle trajectories measured in microscopy experiments contain important information about ...
interrupted. Some in vitro studies have suggested that the intersections of the microtubules are likely to interfere with cargo transport and form tethering points for cargo45. Single-particle tracking combined with confocal microscopy or STORM microscopy has also been employed to investigate vesicle ...
1549 View in ScopusGoogle Scholar 95 A.S. Hansen, M. Woringer, J.B. Grimm, L.D. Lavis, R. Tjian, X. Darzacq Robust model-based analysis of single-particle tracking experiments with Spot-On Elife, 7 (2018), Article e33125 View in ScopusGoogle Scholar Cited by (0)...
Quantitative characterization of changes in dynamical behavior for single-particle tracking studies J. Phys. Chem. B (2006), 10.1021/jp062024j Google Scholar 21 L.P. Watkins, H. Yang Detection of intensity change points in time-resolved single-molecule measurements J. Phys. Chem. B (2005), ...
We have developed new open-source software called cisTEM (computational imaging system for transmission electron microscopy) for the processing of data for high-resolution electron cryo-microscopy and single-particle averaging. cisTEM features a graphical user interface that is used to submit jobs, ...
interrupted. Some in vitro studies have suggested that the intersections of the microtubules are likely to interfere with cargo transport and form tethering points for cargo45. Single-particle tracking combined with confocal microscopy or STORM microscopy has also been employed to investigate vesicle ...
To date, single molecule studies have been reliant on tethering or confinement to achieve long duration and high temporal resolution measurements. Here, we present a 3D single-molecule active real-time tracking method (3D-SMART) which is capable of locki