为了验证鉴定小鼠interneurons的Dlx1/2调控网络,作者分析了人脑的sNuc-Seq(Single nuclei RNA-Seq)数据集。 在人类脑sNuc-Seq数据集上,SCENIC也鉴定出由DLX1/2强烈驱动的interneurons细胞群,该群具有与小鼠相同的motif,并且识别出一组保守的靶标,包括DLX1本身。 接下来,作者将这种跨物种分析扩展到其他细胞类型。与基...
Current best practices in single‐cell RNA‐seq analysis: a tutorialwww.embopress.org/doi/full/10.15252/msb.20188746 单细胞RNA测序(scRNA-seq )是一个飞速发展的领域,该技术帮助科研人员从一堆细胞(bulk)水平精细到单细胞(single-cell)水平研究基因的表达状态,从而可以更加精细的刻画科研对象的分子状态(例...
单细胞RNA测序(single-cell RNA sequencing,scRNA-seq)技术作为一项革命性工具,在单细胞水平上对转录组进行分析,已经被广泛用于多个方面的生物医学研究,包括肿瘤异质性研究、新细胞类型的鉴定、组织发育与细胞分化过程研究和基因调控网络研究等。通过scRNA-seq获得高通量的单细胞转录组数据后,利用原位组织学验证是目前流行...
最厉害,最全,最新的莫过于Sean Davis小哥在Github上建的awesome-single-cell repository资源库: seandavi/awesome-single-cell 针对于初入门者的single-cell RNA-seq数据分析在线课程资料: Analysis of single…
Single-cell transcriptome landscape of zebrafish liver reveals hepatocytes and immune cell interactions in understanding nonalcoholic fatty liver disease. Fish Shellfish Immunol 146, 109428 (2024). Dobin, A. et al. STAR: ultrafast universal RNA-seq aligner. Bioinformatics 29, 15–21 (2013). ...
10,000 single cells were used for single-cell library production, using the Chromium Single Cell 3′ Reagent Kit v3 and the Chromium Controller (10x Genomics, Pleasanton, California, USA) according to the manufacturer’s protocol. Libraries were sequenced on a HiSeq 4000 Sequencer (Illumina, San...
BioSciToolWebsite:Github:亲爱的BioSciTools用户:高校开学已有些时间,此次更新带来基于测序输出结果的单细胞常规转录组(SingleCell RNA-Seq)数据分析程序。多个程序主要参考数据格式和数据分析流程,允许用户执行数据QC、细胞过滤、线粒体基因占比统计、Feature过滤、高变Feature鉴定、PCA分析及JackStraw对PCA的选择、细胞UM...
Single-Cell Profiling of Normal BM Cells, Related to Figure 1 (A) Workflow shows the collection and processing of BM aspirates from healthy donors and AML patients for scRNA-seq. (B) Flow cytometry plots show gating strategy to sort CD34+ and CD34+CD38– populations from healthy BM5 donor...
YeaCellTM1st Strand Synthesis kit for Single Cell 3ʹ RNA-seq利用Oligo (dT)18及TSO Primer引物对单细胞转录本基因进行逆转录,适用于微流控的微量体系。其产物可以经过通用引物进行PCR反应富集cDNA,然后使用翌圣的酶切建库试剂盒,构建测序文库。本产品采用了在M-MLV(RNase H-)Reverse Transcriptase基础上经过多...
Single-cell RNA sequencing (scRNA-seq), a technology that analyzes transcriptomes of complex tissues at single-cell levels, can identify differential gene expression and epigenetic factors caused by mutations in unicellular genomes, as well as new cell-specific markers and cell types. scRNA-seq plays...