The Drosophila eye is a powerful model system to study the dynamics of cell differentiation, cell state transitions, cell maturation, and pattern formation. However, a high-resolution single cell genomics resource that accurately profiles all major cell types of the larval eye disc and their spatio...
Here, we apply an image-based single-cell transcriptomics method, sequential fluorescence in situ hybridization (seqFISH), to detect mRNAs for 387 target genes in tissue sections of mouse embryos at the 8–12 somite stage. By integrating spatial context and multiplexed transcriptional measurements ...
We combined single-cell or single-nucleus RNA sequencing for all biopsies with a panel of four spatial expression assays (Slide-seq, MERFISH, ExSeq and CODEX) and H&E staining of consecutive serial sections from up to 15 of these biopsies. We leveraged the coupled measurements to provide ...
Recent advances in single-cell transcriptomics of the human lung have enabled the identification of new cell types, increased the understanding of differentiation trajectories during lung development and regeneration, and allowed the comprehension of pathological processes from a cell type-specific point of...
Cells are only visible under a microscope, with dimensions between 1-100 micrometers. There are two types of cells: Prokaryotic cell and Eukaryotic cell. A prokaryote is a unicellular organism that lacks a membrane-bound nucleus, mitochondria or any other membrane bound organelle . In prokaryotes...
TgE, target organism enrichment; rDNA, ribosomal DNA; FACS, fluorescent-activated cell sorting; TgBm, target organism biometrics; sc, single cell; MDA, multiple displacement amplification; SGA, start of genome amplification; SAG, single amplified genome; CAG, composite amplified genome; OTU, ...
As an important part of the stomach, gastric antrum secretes gastrin which can regulate acid secretion and gastric emptying. Although most cell types in the gastric antrum are identified, the comparison of cell composition and gene expression in the gast
There were no significant differences in cell size or intercellular spacing between uncleared and postfixed samples in all brain regions assayed (Figure S5B). Whole-Organism PARS Enables Phenotyping and Imaging in an Organ-by-Organ Fashion Following whole-body PARS processing and labeling, major ...
The rotation of the HeLa cells about the x axis could become useful for a thorough scan of the cell, while maintaining the focus of the microscope constant at a certain plane within the cell diameter. It is also worth noting that the trapping and rotation of particles and cells are coupled...
single-cell transcriptomics of the human lung have enabled the identification of new cell types, increased the understanding of differentiation trajectories during lung development and regeneration, and allowed the comprehension of pathological processes from a cell type-specific point of view3,4,5,6,7...