2011. Single-cell dynamics of mast cell-CD4+ CD25+ regulatory T cell interactions. Eur. J. Immunol. 41: 1872-1882.Frossi B, D'Inca F, Crivellato E, Sibilano R, Gri G, Mongillo M, et al. Single- cell dynamics of mast cell-CD4+ CD25+ regulatory T cell interactions. Eur J ...
Prostate cancer is the second most common malignancy in men worldwide and consists of a mixture of tumor and non-tumor cell types. To characterize the prostate cancer tumor microenvironment, we perform single-cell RNA-sequencing on prostate biopsies, prostatectomy specimens, and patient-derived organo...
a, Integration, UMAP and cell populations of 10X multiome ATAC profiles from the Ts21 and disomic datasets. cDC, classical dendritic cell; MEMP, megakaryocyte–erythroid–mast progenitor; pDC, plasmacytoid dendritic cell.b, Circular projection of absorption probabilities for every HSC towards terminal...
single-cell phenotyping, survival and machine learning prediction of clinical outcomes. CyTOF, cytometry by time of flight. Images were created with BioRender.b, Average expression of lineage markers across cell types in the LUAD tissue using the panel of isotope-conjugated antibodies. Cl Mo, class...
一、SummarySingle-cell technologies have revealed the complexity of the tumour immune microenvironment with unparalleled resolution. Most clinical strategies rely on histopathological stratifcation …
单细胞RNA测序(scRNA-seq )是一个飞速发展的领域,该技术帮助科研人员从一堆细胞(bulk)水平精细到单细胞(single-cell)水平研究基因的表达状态,从而可以更加精细的刻画科研对象的分子状态(例如细胞图谱,表达特征等)。目前该领域涌现了一大批用于单细胞RNA测序分析的方法,随着越来越多方法的出现,如何选择和组合使用这些方...
Cutaneous T cell lymphoma (CTCL) represents a heterogeneous group of non-Hodgkin lymphoma distinguished by the presence of clonal malignant T cells. The heterogeneity of malignant T cells and the complex tumor microenvironment remain poorly characterized
Single-cell RNA-sequencing (scRNA-seq) has become a widely used tool for both basic and translational biomedical research. In scRNA-seq data analysis, cell type annotation is an essential but challenging step. In the past few years, several annotation tools have been developed. These methods req...
MAST fits two-part, generalized linear models that are specially adapted for bimodal and/or zero-inflated single cell gene expression data. Examples and vignettes MAST supports: Easy importing, subsetting and manipulation of expression matrices ...
Single-cell RNA sequencing (scRNA-seq), a technology that analyzes transcriptomes of complex tissues at single-cell levels, can identify differential gene expression and epigenetic factors caused by mutations in unicellular genomes, as well as new cell-specific markers and cell types. scRNA-seq plays...