Mast cells are highly versatile in terms of their mode of activation by a host of stimuli and their ability to flexibly release a plethora of biologically highly active mediators. Within the immune system, mast cells can best be designated as an active nexus interlinking innate and adaptive immun...
Effect of signal transduction pathways on the action of thapsigargin on rat mast cells. Crosstalks between cellular signalling and cytosolic pH. Biochem Pharmacol 1994;47:1813±20.Alfonso A, Botana MA, Vieytes MR, LouzaoMC, Botana LM. Effect of signal transduction pathways on the action of ...
In this review, we will focus on cells and cellular networks encompassing monocytes, macrophages, and mast cells that can uniquely amplify DENV infection by ADE and modulate pathogenesis by release of cytokines, chemokines, proteases and other factors. The monocyte-macrophage-mast cell axis Monocytes...
Mast cells (MCs) are immune cells that play roles in both normal and abnormal processes. They have been linked to tumor progression in several types of cancer, including non-small cell lung cancer (NSCLC). However, the exact role of MCs in NSCLC is still
Cellular chymase activity was measured using spectrophotometry to assess degranulation capacity. Log-phase HMC-1 cells and XBP1+ mast cells were digested, resuspended, and counted, with cell concentration adjusted to 5 × 10^6/mL. 1mL of each cell suspension was added to centrifuge tubes ...
The activation of A549 cells by EVs led us to ask whether the relative abundance of various proteins and not just their phosphorylation might be regulated by the signaling networks within the cells. In-silico protein clas- sification revealed a number of proteins that are known to regulate the...
And that’s just the tip of the iceberg. They’re involved insomuch of the body’s processes that patients go to different specialists with seemingly no commonalities within their symptom profile—only to later find out that the mast cells, located all over the body, are the culprit. ...
The origin of the Hofbauer cells is still debated. According to some authors, they derive from mesenchymal cells within the stroma of the chorionic villi [132,133]; according to others, they derive from progenitor monocytes in the yolk sac of the embryo [134,135]. Instead, decidual MΦs ...
Figure 1. MCs are located in zones subjected to cyclic hypoxia within murine B16 melanoma tumors. Three to four weeks after inoculation of B16-F1 cells on mouse ear pinna, tumors were excised and processed as described in the Materials and Methods section. Thirty μm-thick slices were stained...
To determine the total cellular content of this enzyme, an equivalent number of cells were lysed with 1% triton X-100 (Sigma-Aldrich) and the plate blank was deducted. Release of β- hexosaminidase was calculated as the percentage of the total enzyme content. Inflammatory response was assessed ...