Isolation of genomic DNA molecule from a single cell and control its higher order structure using optical tweezersoptical tweezersfluorescence microscopyDNAhigher order structureKOD1S. pombeIn this report, we describe a noninvasive methodology for manipulating single Mb-size whole-genome DNA molecules. ...
Optoelectronic tweezers (OET) has advanced within the past decade to become a promising tool for cell and microparticle manipulation. Its incompatibility with high conductivity media and limited throughput remain two major technical challenges. Here a no
P. In situ mRNA isolation from a microfluidic single-cell array using an external AFM nanoprobe. Lab Chip 17, 1635–1644 (2017). Article CAS Google Scholar Seger, R. A. et al. Voltage controlled nano-injection system for single-cell surgery. Nanoscale 4, 5843–5846 (2012). Article ...
63x objective Leica 506081 Used to locate laser beams spots during alignment and to find focus and X-y position in flow cell; can be used for optical trapping as it has an identical back aperture diameter to the 100X; oil immersion lens Alignment laser Lumentum 1100 series 10mW HeNe laser...
Moreover, each cell has a tiny quantity of DNA, making it challenging to accurately amplify and sequence single cells. Indeed, a long history of methods based on optical tweezers 3 , flow sorting 4 , microfluidics 5,6 , and single cell isolation using gel matrices 7–9 can be used to ...
Capillary-based selective isolation of single cells (MMI CellEctor) Optical tweezers to quantify biological forces (MMI CellManipulator) Our MMI CellCut combines several proven technologies which enable fast and precise laser microdissection of paraffin and cryo samples, smears and live cell cultures. ...
Capillary-based selective isolation of single cells (MMI CellEctor) Optical tweezers to quantify biological forces (MMI CellManipulator) Our MMI CellCut combines several proven technologies which enable fast and precise laser microdissection of paraffin and cryo samples, smears and live cell cultures. To...
A microfluidic device for reversible environmental changes around single cells using optical tweezers for cell selection and positioning Cells naturally exist in a dynamic chemical environment, and therefore it is necessary to study cell behaviour under dynamic stimulation conditions in orde... E Eriksson...
et al. Monitoring the dynamics of primary T cell activation and differentiation using long term live cell imaging in microwell arrays. Lab. Chip 12, 5007–5015 (2012). Article CAS PubMed Google Scholar Harris, T. D. et al. Single-molecule DNA sequencing of a viral genome. Science 320,...
isolation strategies are highly inefficient and typically rely on manual pipetting, which is limited to sorting event rates on the order of 1 cell per minute68. Recently, technologies have come to market that enable the parallelized retrieval of cells from compartments using optical69or optoelectronic...