Single cells can be studied and tracked using many detection technologies, including quantitative imaging and mass spectrometry. However, our Review focuses on single-cell analysis using DNA-sequencing-based te
Single-cell epigenome sequencing for detecting footprints of differentiation of individual cells Single-cell sequencing technologies for studying epigenomics also exist (Table 3). By elucidating the epigenomic status of cells, such as DNA methylation and chromatin states, we can observe the cell lineage...
Next-generation sequencing technologiesWhole genome amplification methodsWhole transcriptome amplification methodsThe cell is the fundamental unit of life, in which the blueprint of the genome is transcribed and translated into biological function. Most of our current understanding of the genome, epigenome,...
normal or diseased tissues, it also provides a cornerstone for studying cell differentiation and development patterns, disease heterogeneity, drug resistance mechanisms, and treatment strategies. Herein, we review traditional single-cell sequencing technologies and outline the latest advancements in single-cel...
Single-cell omics approaches provide high-resolution data on cellular phenotypes, developmental dynamics and communication networks in diverse tissues and conditions. Emerging technologies now measure different modalities of individual cells, such as genomes, epigenomes, transcriptomes and proteomes, in additi...
Recent advances in single-cell RNA sequencing demonstrate the value of a higher resolution view (Sandberg, 2014) and suggest that methods for single-cell epigenome mapping could promote our understanding of epigenetic regulation in development and disease. Whole-genome bisulfite sequencing (WGBS) is ...
6.TAP-seq identifies cell types and differentiation states with shallow sequencing 接下来,因为Single-cell perturbation screens are also used to define changes in cell type abundance and cell state。因为他们这个测序的特点或者是他们认为的优势是:low read depths,To compare the ability of TAP-seq and ...
Here, we profile 36,199 single-cell transcriptomes at multiple time points throughout a highly efficient chemical reprogramming system using RNA-sequencing and reconstruct their progression trajectories. Through identifying sequential molecular events, we reveal that the dynamic early embryonic-like programs...
Cell 169, 1276–1290.e17 (2017). Klein, H.-U. et al. Epigenome-wide study uncovers large-scale changes in histone acetylation driven by tau pathology in aging and Alzheimer's human brains. Nat. Neurosci. 22, 37–46 (2019). López-Sánchez, N. et al. Neuronal tetraploidization in the...
Single cell epigenome sequencing focuses on the heritable changes in phenotype that do not change in the DNA sequence, which involves in DNA methylation, chromatin accessibility, histone modifications, and DNA folding, reflecting how genomic structure variation influences cellular phenotype [57]. ...