Next-generation sequencing technologiesWhole genome amplification methodsWhole transcriptome amplification methodsThe cell is the fundamental unit of life, in which the blueprint of the genome is transcribed and translated into biological function. Most of our current understanding of the genome, epigenome,...
Single-cell epigenome sequencing for detecting footprints of differentiation of individual cells Single-cell sequencing technologies for studying epigenomics also exist (Table 3). By elucidating the epigenomic status of cells, such as DNA methylation and chromatin states, we can observe the cell lineage...
From a single-cell view, the stochastic features of a single cell must be properly integrated into GRN models. As noted above, technical noise is difficult to distinguish from true biological variability, and the remaining variability is still poorly understood. However, the asynchronous nature of ...
normal or diseased tissues, it also provides a cornerstone for studying cell differentiation and development patterns, disease heterogeneity, drug resistance mechanisms, and treatment strategies. Herein, we review traditional single-cell sequencing technologies and outline the latest advancements in single-cel...
We also discuss the potential applications and future directions of these single-cell omics sequencing technologies for different biomedical systems, especially for the human stem cell field. 展开 关键词: single-cell omics genome sequencing epigenome sequencing lineage tracing third-generation sequencing ...
The cell is the fundamental unit of life, in which the blueprint of the genome is transcribed and translated into biological function. Most of our current understanding of the genome, epigenome, and transcriptome is based on analysis of millions of cells as a bulk population. These analyses are...
Single-cell RNA sequencing JEV: Japanese encephalitis virus CNS: Central nervous system DMEM: Dulbecco’s modified Eagle’s medium dpi: Days post infection IHC: Immunohistochemistry FISH: Fluorescence in situ hybridization UMI: Unique molecular identifier ...
Single-cell omics approaches provide high-resolution data on cellular phenotypes, developmental dynamics and communication networks in diverse tissues and conditions. Emerging technologies now measure different modalities of individual cells, such as genomes, epigenomes, transcriptomes and proteomes, in additi...
Recent advances in single-cell RNA sequencing demonstrate the value of a higher resolution view (Sandberg, 2014) and suggest that methods for single-cell epigenome mapping could promote our understanding of epigenetic regulation in development and disease. Whole-genome bisulfite sequencing (WGBS) is ...
To elaborate, an integrated analysis of cancer cells has been shown in hepatocellular carcinoma (HCC) where time-of-flight mass cytometry (CyTOF) and single-cell RNA sequencing were used to show an immunosuppressive gradient of immune cells in a tumour microenvironment, non-tumour microenvironment an...