Single-cell epigenome sequencing techniques have recently been developed. However, the combination of different layers of epigenome sequencing in an individual cell has not yet been achieved. Here, we developed a single-cell multi-omics sequencing techno
Single-cell epigenome sequencing for detecting footprints of differentiation of individual cells Table 3 Single-cell epigenome sequencing. Full size table Proteomics analysis at the single-cell level To comprehensively measure the expression patterns of each protein, researchers generally use mass spectrometry...
Next-generation sequencing technologiesWhole genome amplification methodsWhole transcriptome amplification methodsThe cell is the fundamental unit of life, in which the blueprint of the genome is transcribed and translated into biological function. Most of our current understanding of the genome, epigenome,...
From a single-cell view, the stochastic features of a single cell must be properly integrated into GRN models. As noted above, technical noise is difficult to distinguish from true biological variability, and the remaining variability is still poorly understood. However, the asynchronous nature of ...
With the emergence of single-cell sequencing technology, it has bestowed upon us an unprecedented resolution to comprehend the mechanisms underlying leukemogenesis and drug resistance across various levels, including the genome, epigenome, transcriptome and proteome. Here, we provide an overview of the ...
Single-cell epigenome sequencing techniques have recently been developed. However, the combination of different layers of epigenome sequencing in an indivi... Fan,Guo,Lin,... - 《Cell Research》 被引量: 25发表: 2017年 Single-cell transcriptomics: background, technologies, applications, and challeng...
Recent advances in single-cell technologies have enabled the profiling of the genome, epigenome, transcriptome and proteome, along with temporal and spatia... RHYLF Louie - 《Immunology & Cell Biology》 被引量: 0发表: 2021年 Advances in single-cell sequencing technologies and applications in cance...
Single-cell RNA sequencing JEV: Japanese encephalitis virus CNS: Central nervous system DMEM: Dulbecco’s modified Eagle’s medium dpi: Days post infection IHC: Immunohistochemistry FISH: Fluorescence in situ hybridization UMI: Unique molecular identifier ...
Single-cell omics approaches provide high-resolution data on cellular phenotypes, developmental dynamics and communication networks in diverse tissues and conditions. Emerging technologies now measure different modalities of individual cells, such as genomes, epigenomes, transcriptomes and proteomes, in additi...
automated single-cell lysis, RNA extraction, and cDNA synthesis for up to 800 cells in parallel on a single chip. This platform offers lower false positives and less bias than tube-based technologies. However, its major drawbacks include the number of cells (>1000) required for capture and ...