Here, we combine the CUT&Tag technology, developed to measure bulk histone modifications, with droplet-based single-cell library preparation to produce high-quality single-cell data on chromatin modifications. We apply single-cell CUT&Tag (scCUT&Tag) to tens of thousands of cells of the mouse ...
Probing histone modifications at a single-cell level in thousands of cells has been enabled by technologies such as single-cell CUT&Tag. Here we describe nano-CUT&Tag (nano-CT), which allows simultaneous mapping of up to three epigenomic modaliti
Recent technology development has enabled such profiling at single-cell resolution. However, an end-to-end computational pipeline for analyzing such data is still lacking. To fill this gap, we have developed a flexible pipeline for analysis and visualization of single-cell CUT&RUN and CUT&Tag ...
Single-cell sequencing has shed light on previously inaccessible biological questions from different fields of research, including organism development, immune function, and disease progression. The number of single-cell-based studies increased dramatically over the past decade. Several new methods and tool...
Cell. 2021;184(13):3559–72. Article CAS Google Scholar Deng Y, Bartosovic M, Kukanja P, Zhang D, Liu Y, Su G, Enninful A, Bai Z, Castelo-Branco G, Fan R. Spatial-cut &tag: spatially resolved chromatin modification profiling at the cellular level. Science. 2022;375(6581):681–...
Muscle-invasive bladder cancer (MIBC) is a highly aggressive disease with a poor prognosis. B cells are crucial factors in tumor suppression, and tertiary lymphoid structures (TLSs) facilitate immune cell recruitment to the tumor microenvironment (TME).
Long non-coding RNAs (lncRNAs) comprise a diverse class of transcripts that can regulate molecular and cellular processes in brain development and disease. LncRNAs exhibit cell type- and tissue-specific expression, but little is known about the expressio
--barcodeLevel STRING: 条形码是针对细胞还是分子(默认:cell) --barcodeWhitelist STRING: 条形码白名单路径(默认:不使用) --barcodeTranslate STRING: 条形码转换文件路径(默认:不使用) --UMI STRING: 如果是 -b, 则为10x Genomics类UMI的BAM字段; 如果是 -1 -2/-u, 则为包含10x Genomics类UMI的文件(默认...
[17]. A conceptually similar study has also been performed using the CUT&TAG protocol, in which transposons were used instead of MNase. Many of the techniques derived from the aforementioned protocols have been improved to enable analysis using single-cell DNA input [17,18,19,20]. However, ...
a Single cell CUT&Tag (scCUT&Tag) processing. All steps from antibody incubations through adapter tagmentation are done on a population of permeabilized unfixed cells. Individual cells are then dispensed into nanowells of a Takara ICELL8 chip. After verifying nanowells with single cells by ...