Here, we combine the CUT&Tag technology, developed to measure bulk histone modifications, with droplet-based single-cell library preparation to produce high-quality single-cell data on chromatin modifications. We apply single-cell CUT&Tag (scCUT&Tag) to tens of thousands of cells of the mouse ...
Probing histone modifications at a single-cell level in thousands of cells has been enabled by technologies such as single-cell CUT&Tag. Here we describe nano-CUT&Tag (nano-CT), which allows simultaneous mapping of up to three epigenomic modaliti
Single cell CUT&Tag-pro v1 来自 Semantic Scholar 喜欢 0 阅读量: 4 作者: B Zhang 摘要: scCUT&Tag-pro is a multimodal assay for profiling histone modification coupled with the abundance of surface proteins in single cells. It was developed based on CUT&Tag (Kaya-Okur et al., 2019) and...
Probing histone modifications at a single-cell level in thousands of cells has been enabled by technologies such as single-cell CUT&Tag. Here we describe nano-CUT&Tag (nano-CT), which allows simultaneous mapping of up to three epigenomic modaliti
Paired-Tag,可以同时测 snRNA-seq(核内mRNA) 和 CUT&Tag(蛋白结合)。(大概就是 Paired-seq + CUT&Tag) Image Single-cell profiling of histone modifications in the mouse brain, bioRxiv 2020.09.02.279703 大概做法:使用 Sci 的策略实现单细胞高通量加 Barcode(核心思想就是多轮加 Barcode,从而实现多次 96...
Recent technology development has enabled such profiling at single-cell resolution. However, an end-to-end computational pipeline for analyzing such data is still lacking. To fill this gap, we have developed a flexible pipeline for analysis and visualization of single-cell CUT&RUN and CUT&Tag ...
2019年8月27日,北京大学分子医学研究所,北京大学-清华大学生命科学联合中心研究员何爱彬研究组在Molecular Cell杂志在线发表题为“CoBATCH for High-Throughput Single-Cell Epigenomic Profiling”的文章,报道了一种新的具有普适性、易操作、高通量和高质量的单细胞ChIP-seq技术,并将其命名为CoBATCH(combinatorial barcodin...
Collections of library structure and sequence of popular single cell genomic methods (mainly scRNA-seq). Before you start Make sure you understand the basic configuration of the Illumina libraries, because most single cell sequencing methods are developed to be sequenced on the Illumina platforms. If...
> 0 if there is any Tn5 cut site for each single barcode (i.e. cell) that map within each peak. f_binary_mat <- readMM(file = paste0(cellatac_dir, 'peak_matrix/peaks_bc_matrix.mmtx.gz')) regions.names = read.delim(paste0(cellatac_dir, 'peak_matrix/peaks.txt'), header = ...
which we term single-cell CUT&Tag (scCUT&Tag) (Fig.6a). We performed scCUT&Tag to the H3K27me3 modification on a bulk population of K562 cells, but with gentle centrifugation between steps instead of Concanavalin A magnetic beads. After integration, we used a Takara ICELL8 nano-dispensi...