Sections were labeled with combinations of anti-Ku80 (1:250; Abcam ab79220), anti-Iba1 (1:200; Wako 019–19,741), anti-P2RY12 (1:200; Sigma HPA014518) and mounted with DAPI Fluoromount (SouthernBiotech). Immunofluorescent sections were then visualized and captured using an Olympus FX...
characteristics. To prevent clogging of the flow cell, samples were diluted to 1 ml and acquired using a medium flow rate. Quadrant markers were set on unstained cells for directly labeled mAbs and on cells stained with streptavidin- or avidin-fluorochrome alone ...
Electrophoretic mobility of a labeled PPARgamma response element (PPRE) was retarded in the presence of mesangial cell nuclear extract, suggesting that PPARgamma is functional in these cells. The addition of...
Susceptibility to apoptosis of cultured skin fibroblasts was measured by fluorescent staining of active caspases with the use of the Flica (Fluorescent-Labeled Inhibitor of Caspases) Apoptosis Multi-Caspase Detection Kit (Immunochemistry Technologies), which makes use of an inhibitor sequence of active ...
Sections were labeled with combinations of anti-Ku80 (1:250; Abcam ab79220), anti-Iba1 (1:200; Wako 019–19,741), anti-P2RY12 (1:200; Sigma HPA014518) and mounted with DAPI Fluoromount (SouthernBiotech). Immunofluorescent sections were then visualized and captured using an Olympus FX...
Cell Labeling HiPSCs quickly internalized the prelabeled lipophilic agent. The yield was 22.0% ± 1.8% at 5 min and 42.8% ± 3.8% at 10 min in 3% DMSO samplings, and the bound activity saturated at 15 and 30 min (52.5% ± 2.0% vs. 53.3% ± 2.2%, respectively). The yield was ...
(PD). An increased A2AR availability has been found in the human striatum at early stages of PD and in patients with PD and dyskinesias. The aim of this small animal positron emission tomography/magnetic resonance (PET/MR) imaging study was to investigate whether rotenone-treated mice reflect...