Protein synthesis is one of the most fundamental biological processes, which consumes a significant amount of cellular resources. Despite existence of multiple mathematical models of translation, varying in the level of mechanistical details, surprisingly, there is no basic and simple chemical kinetic ...
A simple, rapid, sensitive, and reproducible cell-free assay system for studying brain protein synthesis is described. This system uses small amounts of brain postmitochondrial supernatant, making it a convenient screening test when only small amounts of tissue are available. It showed over 95% ...
Keratinised stratified squamous epithelium is multi-layered squamous epithelium where the upper layers of cells, furthest from the basement membrane, are no longer alive and are filled with a protein calledkeratin. Keratinised stratified squamous epithelium forms theepidermisof the skin, and a small ...
Muscle protein synthesis rates after ingesting 30 g of potato protein do not differ from rates observed after ingesting an equivalent amount of milk protein. Although you might not eat 30 grams of potato protein at a time, eating white potatoes can positively impact muscle protein synthesis. Addit...
Schematic diagram of char residue layer formation Full size image TG-FTIR TG-FTIR was employed to study the thermal degradation performance of CA/AgCl nanocomposites. Figure 6 shows the 3D FTIR spectrum of the gas generated during the thermal degradation of CA/AgCl nanocomposites, in which the in...
Desulforedoxin is a simple dimeric protein isolated from Desulfovibrio gigas containing a distorted rubredoxin-like center with one iron coordinated by four cysteinyl residues (7.9 kDa with a 36-amino-acid monomer). 1H NMR spectra of the oxidized Dx(Fe3+) and reduced Dx(Fe2+) forms were ...
Another nutrient of concern is protein, but you can get plenty of it from plant foods. Examples include tofu, tempeh, beans, and quinoa. However, if you’d like to supplement your diet, there are many quality vegan protein powders to choose from. FAQ Do vegans really need to take a dai...
Schematic diagram. A 1.65 Kb fragment from intron 10 of the human KCNQ1 gene (dashed arc) was amplified using the primers 3 and 4 and subjected to Flp double cross cloning into receptor plasmid p128lacZ. The resultant recombinants transform XL1-Blue cells to form white colonies (right). ...
dissociate RNA-dependent ribonucleoprotein complexes, 3) protein-RNA complexes are purified and subjected to multiple, stringent washes, 4) proteins are digested and the RNAs are extracted, and 5) RNAs are used for cDNA synthesis which is then used for the construction of the sequencing library. ...
The DNA polyhedra can be used to display protein molecules at designated sites. As an example, maltose-binding protein (MBP) was fused with engineered streptavidin monomer to bind to biotin groups placed at the chosen sites of an octahedron (two sites per edge facing outside of the octahedron...