Microorganisms can be unicellular (single cell), multicellular (cell colony), or acellular (lacking cells). They include bacteria, archaea, fungi, protozoa, algae, and viruses. Bacteria are single-celled microbes that lack a nucleus. Archaea are like bacteria, but they have different structures an...
et al. Normal establishment of epithelial tight junctions in mice and cultured cells lacking expression of ZO-3, a tight-junction MAGUK protein. Mol. Cell. Biol. 26, 9003–9015 (2006). CAS PubMed PubMed Central Google Scholar Guillemot, L. et al. Cingulin is dispensable for epithelial ...
Monitoring agonist-promoted conformational changes of β-arrestin in living cells by intramolecular BRET. EMBO Rep. 6, 334–340 (2005). CAS PubMed PubMed Central Google Scholar Namkung, Y. et al. Monitoring G protein-coupled receptor and β-arrestin trafficking in live cells using enhanced ...
Autophagy is a cellular process that involves the degradation of cellular components, which is triggered in cells during periods of nutrient deprivation or cellular stress [1]. The autophagy process consists of four essential stages: initiation, nucleation, maturation, and degradation [2]. The far-r...
full-length Short Oskar was not localized to the cytoplasm but exclusively in the nucleus in S2R+ cells (Supplementary Fig.1b)28. Therefore, we used the OST4-mCherry-Oskar construct, which localizes to membranous structures in the cytoplasm (Supplementary Fig.1d, e). Upon coexpression, wild...
Autophagy is a cellular process that involves the degradation of cellular components, which is triggered in cells during periods of nutrient deprivation or cellular stress [1]. The autophagy process consists of four essential stages: initiation, nucleation, maturation, and degradation [2]. The far-...
Such interaction domains may control not only the specificity of signal transduction, but also the kinetics with which cells respond to external and intrinsic cues, as discussed below, and can therefore give rise to complex cellular behaviors. Interaction domains were originally identified in the ...
fumigatus. Several CRISPR-Cas9 platforms have been established successfully to edit the genome of A. fumigatus (23–26). However, the current state of the technology relies heavily on DNA-based systems for delivering Cas9 and the gRNA to the nucleus. In these systems, Cas9 and gRNA expression...
We also tested the construct validity of this model by examining for phenotypic differences in the expression of glutamate- related proteins within the nucleus accumbens core. Results: CPP Score was inversely correlated with acute MA- induced locomotor hyperactivity, but positively correlated with the ...
cells in the middle retina, laterally communicated through amacrine cells, then transmitted to ganglion cells in the innermost retina, and then to the brain. In the brain, both achromatic (luminance) and chromatic (color) signals pass through the lateral geniculate nucleus in the thalamus and ...