Protein concentration was determined with QuantiProTM BCA Assay Kit (Cat. No. QPBCA). Using 96-well plates with a U-shape bottom, 150 µg of cell lysate was diluted to 85 µL (final volume) of ddH2O in each well (for background reading, add 85 µL ddH2O only). Each cell ...
Bicinchoninic acid (BCA) assay (DC Protein Assay, Bio-Rad, Hercules, CA, USA) was performed for total protein quantitation of MDM lysates according to the manufacturer’s instructions (Bio-Rad, La Jolla, CA, USA). Samples were assayed in technical replicates and read at 450 nm in a Varios...
In addition to protein determination and quantification in solution, the QuantiPro™ BCA Assay Kit has other applications, including determination of protein covalently bound to agarose supports and protein adsorbed to multiwell plates. It has also been applied to the determination of functional groups...
The expressed fusion protein was purified using Ni-NTA Purification System (Qiagen, Valencia, CA) according to the man- ufacturer's protocol. Protein concentration was quantified using an enhanced BCA protein assay kit (Beyotime Biotechnology, China). Finally, purified sigma C protein was filter-...
In addition to the assay characteristics listed in the protocol, other performance criteria evaluated during the verification process include: cross-reactivity, dilution linearity, kit stability, and sample behavior (for example, detectability and stability).In addition, each panel and kit meet stringent...
The expressed fusion protein was purified using Ni-NTA Purification System (Qiagen, Valencia, CA) according to the manufacturer’s protocol. Protein concentration was quantified using an enhanced BCA protein assay kit (Beyotime Biotechnology, China). Finally, purified sigma C protein was filter-...
Mitochondrial toxicity assay. Mitochondrial toxicity exerted by mSigR1 was measured using the mitochondrial ToxGlo assay kit (Promega: Cat. No–G8000) according to the manufacturer´s protocol. MCF-7 cells were plated on a 96 well plate and transfected with wtSigR1 or mSigR1. 48 h after ...
脂蛋白脂酶 来源于牛奶 ammonium sulfate suspension, ≥2,000 units/mg protein (BCA); CAS Number: 9004-02-8; EC Number: 232-669-1; Synonyms: 二酰基甘油脂(肪)酶,甘油二酯酰水解酶 at Sigma-Aldrich
The QuantiPro™ BCA Assay Kit is used to determine protein concentrations in the range of 0.5-30 μg/mL.