In addition to protein determination and quantification in solution, the QuantiPro™ BCA Assay Kit has other applications, including determination of protein covalently bound to agarose supports and protein adsorbed to multiwell plates. It has also been applied to the determination of functional groups...
Supernatant was collected and assessed for protein concentration using a Pierce Bicinchoninic Acid (BCA) assay (Thermo Scientific, Rockford, IL) per the manufacturers protocol. Samples containing 20 µg of protein were aliquoted for Western Blotting following previously published protocols [ 37 ]. ...
Subsequently, total protein was quantified by a BCA protein assay kit. The membranes were incubated with primary antibodies: S1R (1:1000, DF7363, Affinity), Bcl2 (1:1000, A20777, ABclonal), Bax (1:1000, T40051S, AbmartIP3R (1:1000, A4436, ABclonal), BiP (1:5000, 16396-1-AP, ...
Protein concentration was determined with QuantiProTM BCA Assay Kit (Cat. No. QPBCA). Using 96-well plates with a U-shape bottom, 150 µg of cell lysate was diluted to 85 µL (final volume) of ddH2O in each well (for background reading, add 85 µL ddH2O only). Each cell ...
The expressed fusion protein was purified using Ni-NTA Purification System (Qiagen, Valencia, CA) according to the man- ufacturer's protocol. Protein concentration was quantified using an enhanced BCA protein assay kit (Beyotime Biotechnology, China). Finally, purified sigma C protein was filter-...
ERSE reporter assay showed increased ER stress in both NSC-34 and MCF-7 cells (Figure 1j) expressing mSigR1. Immunoblotting revealed gel top smear (Figure 1k) and significantly increased levels of the ER stress markers GRP78, pEIF2-α, GADD and HSP70 (Figures 1k and l) which correlated ...
The QuantiPro™ BCA Assay Kit is used to determine protein concentrations in the range of 0.5-30 μg/mL.