虽然研究结果表明TRAIL处理对TRAIL- R /SHP-1/Lck复合体有影响,并且与SHP-1磷酸化增强有关,但SHP-1磷酸化对Lck活性的实际作用仍不清楚,需要进一步深入探索以阐明这些微簇成分的空间和动力学关系,以解决TCR复合体、Lck、TRAIL- R和SHP...
SHP-1 regulates Lck-induced phosphatidylinositol 3-kinase phosphorylation and activity. J. Biol. Chem. 274: 27583-27589.Cuevas,B,Lu,Y,Watt,S,Kumar,R,Zhang,J,Siminovitch,KA,Mills,GB.SHP-1 regulates Lck-induced phosphatidylinositol 3-kinase phosphorylation and activity.Journal of Biological ...
因此,TAOK3对SHP-1丰度的调节作为T细胞受体(TCR)信号的调节器,决定了T淋巴细胞的激活阈值。 研究结果表明,TAOK3是一种对TCR信号至关重要的丝氨酸和苏氨酸激酶。它的缺失导致TCR信号减少,并且酪氨酸磷酸酶SHP-1与激酶LCK的相互作用增加,LCK是TCR信号的关键下游介质(图1B)。 图1.A:SHP-1-TAOK3相互作用模型结...
4.4.1培养箱外壳必需有效接地,保证使用安全。 4.4.2培养箱应放置在具有良好通风条件的室内,在其周围不可放置易燃易爆物品,并与墙壁保持10厘米以上的距离。 4.4.3培养箱内物品放置切勿过挤,必须留出空间。 4.4.4当不使用设备时,应切断外来电源。 4.5注意事项 4.5.1控温仪参数循环设定中P、I、D、T、LCK功能的各...
process (2). shp-1 has been involved in the negative regulation of various growth promoting receptors including receptor tyrosine kinase (c-kit, csf-1, egf), cytokine receptors (il-3, ifn-a/b) and other tyrosine based receptor cd22, b & t cell ag receptor, lck, zap-70 and vav (3...
t cell costimulation mediated bycd28and 4-1bb is essential for car-t cell-inducedtumor regression. however,cd28and 4-1bb differentially modulate kinetics, metabolism and persistence of car-t cells, and the mechanisms governing these differences are not fully understood. we found that lck recruited...
t细胞通过tcr-mhc复合物识别抗原之后,胞内大量信号分子会被lck、zap70等激酶磷酸化而激活,参与信号的传递。然而为了维持稳态、防止t细胞的过度激活,t细胞中大量抑制性磷酸酶也会被激活,造成信号分子的去磷酸化而失活,影响了t细胞激活信号的传递,最终削弱了t细胞的杀伤功能[2]。细胞中有一百多种ptp磷酸酶,即使人类...
we identify THEMIS as an uncharacterized substrate for the phosphatase SHP1. Saturated mutagenesis assays and mass spectrometry analysis reveal that phosphorylation of THEMIS at the evolutionally conserved Tyr34 residue is oppositely regulated by SHP1 and the kinase LCK. Similar toTHEMIS−/−mice,TH...
SHP-1inhibitsLPS-mediatedTNFandiNOSproductioninmurinemacrophagesAmyO.Hardina,b,c,ElizabethA.Mealsa,b,TaolinYid,KatherineM.Knappb,e,B.KeithEnglisha,b,*aChildren’sFoundationResearchCenteratLeBonheurChildren’sMedicalCenter,Room301WestPatientTower,50NorthDunlap,Memphis,TN38103,USAbDepartmentofPediatrics,Uni...
Study of Lck- and FynT-dependent T cell activation. J Biol Chem. 1996, 271 (2): 695-701. 10.1074/jbc.271.2.695. Article PubMed CAS Google Scholar Zhang SQ, Yang W, Kontaridis MI, Bivona TG, Wen G, Araki T, Luo J, Thompson JA, Schraven BL, Philips MR, Neel BG: Shp2 ...