虽然研究结果表明TRAIL处理对TRAIL- R /SHP-1/Lck复合体有影响,并且与SHP-1磷酸化增强有关,但SHP-1磷酸化对Lck活性的实际作用仍不清楚,需要进一步深入探索以阐明这些微簇成分的空间和动力学关系,以解决TCR复合体、Lck、TRAIL- R和SHP...
因此,TAOK3对SHP-1丰度的调节作为T细胞受体(TCR)信号的调节器,决定了T淋巴细胞的激活阈值。 研究结果表明,TAOK3是一种对TCR信号至关重要的丝氨酸和苏氨酸激酶。它的缺失导致TCR信号减少,并且酪氨酸磷酸酶SHP-1与激酶LCK的相互作用增加,LCK是TCR信号的关键下游介质(图1B)。 图1.A:SHP-1-TAOK3相互作用模型结...
Conclusions: TRAIL-R associates with phosphatase SHP-1 and transduces a unique and distinct immune gatekeeper signal to repress TCR signaling and T cell activation via inactivating Lck. Thus, our results define TRAIL-R as a new class of immune checkpoint receptors for rest...
因此,TAOK3对SHP-1丰度的调节作为T细胞受体(TCR)信号的调节器,决定了T淋巴细胞的激活阈值。 研究结果表明,TAOK3是一种对TCR信号至关重要的丝氨酸和苏氨酸激酶。它的缺失导致TCR信号减少,并且酪氨酸磷酸酶SHP-1与激酶LCK的相互作用增加,LCK是TCR信号的关键下游介质(图1B)。 图1.A:SHP-1-TAOK3相互作用模型结...
因此,TAOK3对SHP-1丰度的调节作为T细胞受体(TCR)信号的调节器,决定了T淋巴细胞的激活阈值。 研究结果表明,TAOK3是一种对TCR信号至关重要的丝氨酸和苏氨酸激酶。它的缺失导致TCR信号减少,并且酪氨酸磷酸酶SHP-1与激酶LCK的相互作用增加,LCK是TCR信号的关键下游介质(图1B)。 > 图1. A:SHP-1-TAOK3相互...
Expression of CD45 alters phosphorylation of the lck-encoded tyrosine protein kinase in murine lymphoma T-cell lines CD45 is a family of high molecular weight leukocyte cell surface glycoproteins. Recently, two related subregions of the cytoplasmic domain of CD45 have bee... HL Ostergaard,DA ...
Remarkably, a KI mutation of LCK Ser59, previously suggested to be key in ERK-mediated resistance towards SHP1 negative feedback, did not affect TCR signalling nor ligand discrimination in vivo . Thus, the THEMIS:SHP complex dampens early TCR signalling by a previously unknown molecular ...
THEMIS-SHP1 Counteracts the Effect of LCK in the CAR Synapse of CAR-T Cells Encoding 4-1BB Although co-receptors bring LCK into the CAR19.BBζ synapse (Figures 3D–3F and S3F; Table S2), we observed very low basal phosphorylation of the CAR-CD3ζ, suggesting that 4-1BB may recruit...
we identify THEMIS as an uncharacterized substrate for the phosphatase SHP1. Saturated mutagenesis assays and mass spectrometry analysis reveal that phosphorylation of THEMIS at the evolutionally conserved Tyr34 residue is oppositely regulated by SHP1 and the kinase LCK. Similar toTHEMIS−/−mice,TH...
1e)24. To generate T cells lacking all PKD isoforms, we established T cell-specific PKD2/PKD3 double-deficient mice using Lck-Cre Tg mice (PKD2/3ΔT) (Fig. 2a,b). We confirmed that none of the three PKD isoforms was expressed in PKD2/3ΔT thymocytes using Abs reacting with PKD1, ...