The invention discloses a multiplex PCR detection method and kit for Shiga toxin-producing Escherichia coli and application thereof. The method and the kit thereof can simultaneously detect the genes consisting of stx1, stx2 and wzyO157 through a triplex PCR reaction, electrophoresis staining and ...
coli O157:H7 test kit (Oxoid). Isolates were then confirmed as E. coli using the Microbact 12E or 24E system (Oxoid). Additional attempts were made to isolate E. coli O157 from all 1,500 samples using a method similar to that of Fegan et al. (14). Briefly, IMS was performed on ...
中文名称:志贺样毒素(SLT)ELISA试剂盒 中文同义词:志贺样毒素(SLT)ELISA试剂盒 英文名称:Shiga-like toxin (SLT) ELISA kit 英文同义词:SLT CAS号: 分子式: 分子量:0 EINECS号: 信息错误报告 您的Email: 按国家浏览Shiga-like toxin (SLT) ELISA kit国外供应商中国供应商 ...
Residual endotoxin was assayed with Limulus amoebocyte lysate (PYROGENT Plus test kit; Cambrex) or QCL-1000 chromogenic LAL end point assay kit (Lonza Walkersville) and was < 1 ng/mg of protein. Endothelial cell culture and quantitation of VWF strings Pooled donor HUVECs (Lonza Walkerville) ...
Enterohemolysin or Enterohaemorrhagic Escherichia coli; Ehly: Hemolysin; HC: Hemorrhagic Colitis; HUS: Hemolytic Uremic Syndrome; PCR: Polymerase Chain Reaction; SPSS: Statistical Package for the Social Sciences; STEC: Shiga toxin producing Escherichia coli; Stx: Shiga toxin Acknowledgements The authors ...
kit.The antigenic epitopes that can be recognized by STX2-1A3,STX2-1E10 and STX2-3A7 were analyzed by the ELISA additivity test.Results Three hybridoma cell strains were obtained and named as STX2-1A3,STX2-1E10 and STX2-3A7,respectively,all of which produced monoclonal antibodies ...
(0.030 ± 0.001) × 106;p< 0.005,t-test) with respect to controls (see above) when the toxin was administered alone (Figure 5). By contrast, the combined treatment of Raji cells with an inhibitor of DNA repair (Stx1) and an alkylating agent (mafosfamide) significantly (p< 0.005, ...
blindness and neurological abnormalities. Although numerous studies have defined apoptotic responses to Shiga toxin type 1 (Stx1) or Shiga toxin type 2 (Stx2) in a variety of cell types, the potential significance of Stx-induced apoptosis of photoreceptor and pigmented cells of the eye following ...
BioControl, Molecular based PCR test for the detection of Shiga Toxin genes in "Top Seven" Shiga Toxigenic E. coli (STEC) in food Sign Into View Organizational & Contract PricingPricing and availability is not currently available.Recommended Products Slide 1 of 10 Sigma-Aldrich T6557 Anti-γ-Tu...
International Journal of Nanomedicine Dovepress open access to scientific and medical research Open Access Full Text Article ORIGINAL RESEARCH Shiga Toxin-B Targeted Gold Nanorods for Local Photothermal Treatment in Oral Cancer Clinical Samples Elena Navarro-Palomares1, Lorena García-Hevia 1, Jesús ...