We evaluated the SHIGA TOXIN QUIK CHEK (STQC) on its suitability for Shiga toxin-producing Escherichia coli (STEC) testing on human fecal samples after overnight enrichment. Our in-house PCR-based protocol for STEC detection was used as the standard for comparison. STQC detected all described ...
In this study we evaluated the performance of the SHIGA TOXIN QUIK CHEK (Techlab庐, Blacksburg, VA) and the ImmunoCard STAT! Enterohaemorrhagic E. coli (EHEC) (Meridian BioScience, Cincinnati, OH, USA) assays as methods for qualitatively detecting the presence of Shiga toxin in human fecal ...
Alere SHIGA TOXIN QUIK CHEK™•All Stx subtypes except Stx2f were detected with the SHIGA TOXIN QUIK CHEK.•Specificity was 100% for all test methods.•Sensitivity of direct fecal testing was unacceptably low (46.7%).•Culture or enrichment of the stool should always be performed before...
All 26 S. flexneri isolates carried the stx1a gene subtype and expressed the toxin as detected by the SHIGA TOXIN QUIK CHEK™ assay. Table 1. stx1 positive S. flexneri strains and associated demographic data. 2.2. Sporadic Cases of stx1 Positive S. flexneri in Alberta, Canada Among the...