Microtubule organization is determined by the shape of epithelial cellsInterphase microtubule organization is critical for cell function and tissue architecture. In general, physical mechanisms are sufficient to drive microtubule organization in single cells, whereas cells within tissues are thought to ...
Here, we develop a minimal continuum model of a planar epithelial tissue as an active elastic material that incorporates all these features. We start from a full three-dimensional (3D)description of the tissue and derive an effective 2D model that captures, through the curvature of the apical ...
Phenotypic state was first assessed using RT-PCR to quantify mRNA levels of eleven genes associated with physiological function, selected using gene ontology terms related to glomerular epithelial differentiation and physiology19,20,21,22. Compared to unpatterned cells, podocytes cultured on 3-D biochip...
They did this by looking at epithelial cells in the salivary glands ofDrosophilalarvae, where they found that similar shapes existed there. They saw that up to a certain radius of a tube-like shape, the cells would assume a frustum structure. On making the tube more asymmetric (the outer r...
the researchers were able to guide the stem cells to grow into the epithelial tissues with realistic crypt and villi—"peaks and valleys"—anatomy of the real organ. This is particularly important, as much of the functionality of the intestine depends on the different cell populations residing in...
Tissue morphogenesis requires the coordinated regulation of cellular behavior, which includes the orientation of cell division that defines the position of daughter cells in the tissue. Cell division orientation is instructed by biochemical and mechanical signals from the local tissue environment, but how...
- 《Cell & Tissue Research》 被引量: 90发表: 1999年 Proliferation and differentiation of midgut epithelial cells from Manduca sexta, in vitro We have developed an insect midgut primary cell culture from pharate fourth instar larvae of Manduca sexta. An enriched Grace''s medium supplemented with...
multiplein vitrostudies also suggest a role for cell shape in regulating cell fate specification. For example, in murine small intestinalorganoids, the cellular aspect ratio is found to be a keyphysical parameterfor patterning epithelial cell progeny [36]. Moreover, micropatterning of single cells ...
Our findings argue that IRSp53 controls epithelial tissue polarity and lumenogenesis in vitro through the regulation of polarized trafficking and by acting as a scaffold for the assembly of apically restricted RAB35 and EPS8 complexes (Fig.6e). However, IRSp53 can also sense and control membrane...
Fine structure of a third epithelial component of the thyroid gland of the rat. Duct-like structures made of cells different from follicular or light cells were observed with the electron microscope in the thyroid glands of 16 rats. Th... R Calvert,H Isler - J.P. Tarcher ; 被引量: 17...