一、sgRNA文库介绍 sgRNA文库是指由大量单导RNA(single-guide RNA, sgRNA)序列组成的集合,这些sgRNA序列靶向特定基因组区域并用于CRISPR-Cas9系统中的基因编辑实验。 1、高通量筛选:sgRNA文库通常包含成千上万个sgRNA序列,能够同时靶向多个基因,通过高通量筛选找到影响特定表型的关键基因。在设计文库时,通常针对每个基因...
CRISPR筛选(CRISPR-Screen)是一种结合了CRISPR-Cas9系统和高通量筛选的实验技术。CRISPR-Screening基于CRISPR-Cas9系统,通过构建sgRNA文库同时对多个细胞的不同基因进行编辑,再辅以特定的筛选方案,通过 NGS 测序和生信分析的手段,筛选出符合条件的候选基因。CRISPR-Screening已被广泛地运用于药物筛选、鉴定信号通路组分、探究...
Performing a phenotypic screen using the Guide-it CRISPR Genome-Wide sgRNA Library System CRISPR screening webinar Lentiviral CRISPR/Cas9 knockout screening made simple 实验例: 图1 Guide-it CRISPR sgRNA文库所使用的载体和sgRNA scaffold示意图。Panel A.从pLVXS-EF1a-Cas9-PGK-Puro和pLVXS-sgRNA-mCherry...
Clontech Guide-it sgRNA Screening Kit说明书 Comparison of mutation detection assays for detecting NHEJ- C A B Scan to learn more about Clontech’s solutions for CRISPR/Cas9 genome engineering sgRNA sgRNA None % Knockdown Control 52.3 36.5 T1 T2 Visit us at www.clontech.com ...
为加快构建进度,保证编辑正确率和靶点命中率,擎科生物推出人全基因组、特定信号通路或客户指定基因的sgRNA文库构建及筛选的一站式解决方案,包括sgRNA文库设计、sgRNA合成、慢病毒包装、细胞株构建、CRISPR Screen(sgRNA文库)测序分析等服务,为sgRNA文库构建保驾护航。 1、sgRNA文库构建 (1)产品详情 (2)交付标准 1)...
Performing a phenotypic screen using the Guide-it CRISPR Genome-Wide sgRNA Library System CRISPR screening webinar Lentiviral CRISPR/Cas9 knockout screening made simple 实验例: 图1 Guide-it CRISPR sgRNA文库所使用的载体和sgRNA scaffold示意图。Panel A. 从pLVXS-EF1a-Cas9-PGK-Puro和pLVXS-sgRNA-mCherr...
两种方式呈现处理组和对照组之间,在目的基因上,不同的sgRNA富集变化情况。 1. sgr2<-ggplot()+geom_point(aes(x=index,y=LFC,color=type),sgdf,size=2)+ scale_color_manual(name='', values = c('blue','orange'), breaks = c('sig','nt'), labels=c('sg_Ap5b1','sg_NT'))+geom_point(...
Mei, H., et al., Surfaceome CRISPR screen identifies OLFML3 as a rhinovirus-inducible IFN antagonist. Genome Biol, 2021.22(1): p. 297. Concordet, J.P. and M. Haeussler, CRISPOR: intuitive guide selection for CRISPR...
Overview of a genome-wide sgRNA library screen What are some possible methods for performing a genome-wide screen and points to consider? Using a drug resistance phenotype screen as an example, learn how knocked-out genes that convey resistance or sensitivity to the drug can be identified ...
[6] Li H, Liu Y, Xiao Y, et al. CRISPR metabolic screen identifies ATM and KEAP1 as targetable genetic vulnerabilities in solid tumors[J]. Proceedings of the National Academy of Sciences, 2023, 120(6): e2212072120. [7] Schneider W M, Luna J M, Hoffmann H H, et al. Genome-scal...