中文名称:GUIDE-IT™ COMPLETE SGRNA SCREENING SYSTEM 中文同义词:GUIDE-IT? COMPLETE SGRNA SCREENING SYSTEM 英文名称:Guide-it™ Complete sgRNA Screening System 英文同义词:Guide-it? Complete sgRNA Screening System CAS号: 分子式
High-quality gRNAs for CRISPR-Cas9 and screening applications When using the CRISPR-Cas9 system to knock out gene expression or knock in a specific mutation, the design, production, and delivery of high-quality guide RNAs (gRNAs) are critical to success. Whether you need transfection-ready gRNAs...
An sgRNA combines both RNAs into a single, long RNA. sgRNAs are typically 100 nt in length and can be expressed from a vector or chemically synthesized. Because the modified sgRNA demonstrates superior efficiency across more cell types than the two-part system, the TrueGuide crRNA:tracrRNA two...
As the dual-CRISPR system aims to delete the entire NCREs, it is not able to pinpoint the exact functional units within the NCRE, compared with other complementary CRISPRi systems, which tile the defined regions using an array of single-guide RNAs90,91. This dual-CRISPR screening system has...
In all cases, the Guide-it Long ssDNA Production System v2 generated a cleaner band of ssDNA than the previous version of the kit, suggesting more complete and uniform digestion. *Please note that with the launch of the Guide-it Long ssDNA Production System v2, the original Guide-it ssDNA ...
3. Introduction of CRISPR reagents into your system, using best practices for your system. 4. Appropriate selection and screening of candidate genome edits. 5. Verification of your genome edit. When performing non-templated genome editing, you need to verify that your edit occurred, determine the...
2.1RNA Polymerase II Promoters Are Not Suitable for Direct sgRNA Production GuideRNAsneed to meet several requirements in order to edit genes successfully. First, a sgRNA has to be localized in the nucleus. Otherwise, it cannot bring Cas9 to the target DNA to generateDSB, which is a prerequisi...
the aim of providing a highly effective, user-friendly system for performing genome-wide phenotypic screens in human cell lines, we have applied the latest research regarding sgRNA library design and our expertise in lentiviral systems to develop theGuide-it CRISPR Genom...
The invention discloses an efficient specific sgRNA recognition site guide sequence for pig gene editing and a screening method thereof. The screening method includes: screening functional genes, performing ORF analysis, predicting a functional gene sgRNA recognition site guide sequence, detecting whole-...
CRISPR-Cas9 screening A549 cells expanded from a single clone harboring a doxycycline-inducible Cas9 (Addgene #114010) were a gift from J.T. Poirier [34]. To ensure that most cells harbor a single sgRNA integration event, the volume of viral supernatant that would achieve an MOI of\(\appro...