Cpf1 sgRNA设计软件sgRNA Scorer 2.0，都需要自行编写代码，也有一些在线分析软件如CHOPCHOP。
靶向miRNA前体不同类型sgRNA的丰度及特异性-遗传.PDF,Hereditas (Beijing) 2018 年 7 月, 40(7): 561 ―571 研究报告 靶向miRNA 前体不同类型sgRNA 的丰度及 特异性评估 1 1 1 2 1 1 1 1 刘海龙 ,谌阳 ,高杨 ,周玲 ,韩晓松 ,赵长志 ,杨高娟 ,陈毅龙 , 杨慧1 ,谢胜
M. sgRNA Scorer 2.0: a species-independent model to predict CRISPR/Cas9 activity. ACS Synth. Biol. 6, 902–904 (2017). CAS PubMed PubMed Central Google Scholar Singh, R., Kuscu, C., Quinlan, A., Qi, Y. & Adli, M. Cas9-chromatin binding information enables more accurate CRISPR ...
73Citations 52Altmetric Metrics Abstract CRISPR–Cas9 screens have emerged as a transformative approach to systematically probe gene functions. The quality and success of these screens depends on the frequencies of loss-of-function alleles, particularly in negative-selection screens widely applied for pro...
The adaptive immunity system in bacteria and archaea, Clustered Regularly Interspaced Short Palindromic Repeats, CRISPR-associate (CRISPR/Cas), has been adapted as a powerful gene editing tool and got a broad application in genome research field due to its ease of use and cost-effectiveness. The ...
Chari, R.; Yeo, N.C.; Chavez, A.; Church, G.M. sgRNA Scorer 2.0: A Species-Independent Model To Predict CRISPR/Cas9 Activity.ACS Synth. Biol.2017,6, 902–904. [Google Scholar] [CrossRef] [Green Version] Xu, H.; Xiao, T.; Chen, C.-H.; Li, W.; Meyer, C.A.; Wu, Q...