The Sf9 cell line was derived from pupal ovariantissue of the Fall armyworm Spodoptera frugiperda.The Sf9 cell line is highly susceptible to infection with Autographa california nuclear polyhedrosis virus(AcNPV baculovirus), and can be used with allbaculovirus expression vectors. Sf9 cells are...
In this work, an Sf9 cell fed-batch process was developed in shake flasks. The maximum cell density was 28.6 × 106 cells/mL, which was 119% greater than that of the batch process. rAAV was produced on the basis of this cell culture process; the maximum cell density reached 22...
Insect Cell Culture Brochure Insert BaculoDirect Bac. Expr. Sys. Product Information Product Sheet: Sf9 cells in Sf-900 II SFM and Sf9 cells in Sf-900 III SFM 有限使用商标许可 ' Notice to Purchaser: The purchase of this product conveys to the purchaser the limited, non-transferable right ...
Insect Cell Culture Brochure Insert Product Information Product Sheet: Sf9 cells in Sf-900 II SFM and Sf9 cells in Sf-900 III SFM 有限使用商标许可 ' Notice to Purchaser: The purchase of this product conveys to the purchaser the limited, non-transferable right to use the purchased amount of...
Fig.3 Sf9cellgrowthinculture with different concentr a- tion of serum 3. 2 血清梯度实验 Sf9 细胞对于含8%血清的培养液适应很快, 1 代之后即可良好生长; 适应5%的血清需传 代2 次, 共12d; 适应2%的血清较慢, 共需3 代18d; 由2%的血清到无血清培养液适应较 快, 需2 代10d. Sf9 细胞在不同血...
本实验采用SF9悬浮细胞在CloudReady™ 500mL云平台平行生物反应器系统上进行了批次(Batch)培养,最终活细胞密度(Viable cell density,VCD)达到1.20×107cells/mL,活率(Viability)维持在95%以上。验证了CloudReady™能够用于SF9悬浮细胞培养,其稳定、智能的参数控制可以满足SF9悬浮细胞在各个工艺上的条件优化,详见下文。
Key words Pollution black dots cell culture 前言 污染是细胞培养的大敌。预防和避免污染是细胞培养成功的关键之一。一开始就要十分重视，防止污染，否则会前功尽弃，不仅浪费时间，而且浪费人力、物力，甚至造成无法弥补的损失。细胞培养中常见的生物污染类型有7种，分别是细菌污染、支原体污染、原虫污染、黑胶虫污染、...
5. 冷冻保护剂浓度为5 %或10 % DMSO,若是不确定细胞之冷冻条件,在做冷冻保存之同时,亦应作一个backup culture,以防止冷冻失败。 Adenosine deaminase中文名称: 腺苷脱氨酶(ADA)抗体英文别名: ADA; ADA1; ADA_HUMAN; Adenosine aminohydrolase;交叉反应: Human, Mouse, Rat, Pig, Cow, Horse标记: Unconjugated...
grace培养基 Grace’s Insect Cell Culture Medium 是无血清培养基,使用时需要补充血清,从而为细胞提供必要的营养因子。 SF培养基这个名字很常见,举例,义翘的SF培养基(SIM SF)是无血清即用型培养基,不需要另行添加血清组分,可以直接用于SF9和SF21细胞的培养。我...
SF-1, a low cost culture medium for the production of recombinant proteins in baculovirus infected insect cells Two new cell culture media for the insect cell lines Spodoptera frugiperda, Sf9 and Sf21 are described which are useful for the production of recombinant p... EJ Schlaeger,M Fogget...