StringTie 2.2.1toolkit.StringTieis a fast and highly efficient assembler of RNA-Seq alignments into potential transcripts.StringTie Mergetool merges/assembles GTF/GFF transcript files into a non-redundant set of transcripts. This tool should be used afterStringTietranscript assembling of each sample in...
Shubarna joins Vintage Wine Estates with extensive experience in the fast-moving consumer goods and nutrition industries. Previously, Shubarna served as the global director for the center of excellence at Unilever LTD. In her new role, Shubarna will help Vintage Wine Estates drive greater efficiency...
NovaSeq was performed using total RNA from the seven cell lines–BJ fibroblasts, MCCs MKL-1 and MS-1, colorectal carcinomas Hct-116 and Caco2, and pancreatic carcinomas MiaPaca2 and Capan2. Bioinformatic analysis was conducted using the generated sequence reads as outlined in Fig. 1. Total ...
www.nature.com/scientificreports OPEN received: 19 June 2015 accepted: 06 November 2015 Published: 08 December 2015 Seven gene deletions in seven days: Fast generation of Escherichia coli strains tolerant to acetate and osmotic stress Sheila I. Jensen, Rebecca M. Lennen, Markus J....
DNA Extraction and Library Construction DNA extraction was performed using QIAamp® Fast DNA Stool Mini Kit (QIAGEN, Germany) following the manufac- turer's instructions. However, the mucus sample was diluted if the pipetting of the sample is hard. The library construc- tion was followed ...
FFT Fast Fourier transform MPC Micro processor controller THD Total harmonic distortion Appendix A System Voltage 132 kV Transformers 100 MVA, 50 Hz, Tr 1: 25.4 kV/2.12 kV, Tr 2: 50.8 kV/2.12 kV DC-Link Voltage 3000 V Capacitor 0.07 F VSC Power Circuits 02 No. of GTOs 24 GTO’s pul...
RNA/GIT mixtures were layered on top of CsCl and centrifuged at 35,000 rpm (179,000×g) for 21 hours. RNA pellets were resuspended in H2O, ethanol precipitated, and treated with Proteinase K to remove any RNase contamination. After a phenol/chloroform extraction, the RNA was reprecipitated,...
This study performed a genome-wide comparative analysis of theLEAgene family across sevenIpomoeaspecies. A total of 73, 64, 77, 62, 70, 70, and 74LEAgenes were identified in sweet potato (I. batatas),I. trifida,I. triloba,I. nil,I. purpurea,I. cairica, andI. aquatica, respectively. ...
There was no additional benefit of adding NAs to IFN therapy for chronic hepatitis D; however, the combination of IFN + BLV significantly improved short-term HDV RNA clearance, which showed strong synergistic effects. The seven regimens included in the study did not ...
RNA editing events have been proved universally in CP genomes since first reported54. Regarding the current CP genomes, a total of 56 potential RNA editing sites from 32 genes were predicted in each species (Supplementary TableS6). In all seven CP genomes, the event of S converting to L occ...