Serum and Plasma are essential components of blood. In this blog we compare how to collect both sample types and how to measure analytes in these sample types via ELISA.
Blood phosphorylated tau (p-tau) forms are promising Alzheimer’s disease (AD) biomarkers, but validation in matrices other than ethylenediaminetetraacetic acid (EDTA) plasma is limited. Firstly, we assessed the diagnostic potential of p-tau231 and p-tau
When a paraprotein forms dimers, pentamers, polymers, or aggregates with each other, or when forming complexes with other plasma components, a broad smear may be visible instead of a distinct band. ▪ Some patients may produce only light chains, which are rapidly excreted in the urine, and...
Proteomic analysis of plasma sEVs reveals that TNFAIP8 is a new biomarker of cell proliferation in diabetic retinopathy 10.1021/acs.jproteome.0c01048 View article View in Scopus Google Scholar [24] K. Chen, M. Sheng, J. Zhang, G. Yan, B. Li ...
aFITC-labeled AF conidia (1.6 × 106) phagocytosis by freshly isolated human neutrophils (2 × 105) in presence of sera depleted (−) of complement components and the effect of human SAP opsonisation after 1 and 30 min. AF conidia (2 × 108) opsonisation with 100 ...
Plasma lipase activity was determined by the titrimetric method, also using a commercial kit (Bioassay), following the manufacturer's instructions. The principle of this method is that the degradation of triolein by lipase releases diacylglycerol, which reacts further, eventually forming hydrogen peroxi...
[32]. One of the main plasma components leaking through destructed BBB is albumin, which is the most abundant protein in the plasma, and plays a vital role in transporting metabolites, nutrition, and maintaining colloid osmotic pressure. BBB disruption increases albumin levels in the cerebrospinal ...
Again, the proportion of small RNA components varied among species. Interestingly, although the amount of small RNA in human sEVs and sEV-depleted serum was the lowest (Fig.2C), their proportion of miRNAs was the highest among the three species (Fig.3). In contrast, tRNAs (tRFs & tiRNAs...
Published comparisons of serum, plasma, and tear components highlight similarities between these biofluids but do not provide ranges to gauge the bio-variability of each constituent within Auto-SED vs the natural tear film. The composition of the Auto-SEDs made from donations from patients with ...
Recent studies demonstrated that miRNAs are not confined within cells of origin but can be also secreted, thus being found in different biological fluids such as serum or plasma [21]. In serum/plasma they can be found associated with different cellular components such as protein-bound miRNAs (...