All serum samples were analyzed at the University Hospital Basel, Switzerland. Serum NfL levels were determined by single molecule array (Simoa) assay using the capture monoclonal antibody (mAB) 47:3 (initial dilution 0.3 mg/mL; Art. No. 27016) and the biotinylated detector mAB 2:1 (0....
In the investigation of our results, the biological processes (Fig. 1B), cellular components (Fig. 1C), molecular functions (Fig. 1D) and pathways corresponding to differentially secreted serum proteins (Fig. 1E) were analyzed. Finally, a protein-protein interaction network, consisting of all ...
Evaluation of Production and Characterization of Monoclonal Antibodies to Human IgG of Four Subclasses Human IgG of four subclasses, semi-purified from pooled human serum by a series of DEAE ion exchange and protein A affinity chromatographies, were used as immunogens and initial screening antigens...
[15]. Furthermore, it has been shown that this assay may also cross-react with proteins that have a similar structure to sclerostin, which was evidenced by the detection of low “sclerostin” protein levels in the serum of sclerosteosis patients (i.e., patients in which functional or ...
the most common β migrating monoclonal protein, monoclonal IgM and IgG can also be seen. As such, careful review is necessary to eliminate this possibility during visual inspection. Triggers for further investigation includehypogammaglobulinemiawith or without increased or unusualβ globulins(Fig. 5...
About 15% of monoclonal components or paraproteins are associated with malignancy when detected by simple electrophoresis on cellulose acetate membranes or agarose gel followed by protein staining. More sensitive methods for detecting monoclonal components result in a lower frequency of association with ...
NK1.1 cells express 4-1BB (CDw137) costimulatory molecule and are required for tumor immunity elicited by anti-4-1BB monoclonal antibodies. Cell Immunol 1998;190:167-72 Metkar SS, Naresh KN, Manna PP, Srinivas V, Advani SH, Nadkarni JJ. Circulating levels of TNF alpha and TNF receptor ...
A method for preparing serum depleted of IgG to be used in the production of IgG monoclonal antibodies is disclosed. Animal serum is contacted with protein G or protein A prior to addition to base media to obtain a superior cell culture medium depleted of serum derived IgG antibodies. Included...
The clinical efficacy of type 2 monoclonal antibodies in eosinophil-associated chronic airway diseases: a meta-analysis. Front Immunol. 2023;14:1089710.10.3389/fimmu.2023.1089710Search in Google Scholar PubMed PubMed Central [71] Veerati PC, Mitchel JA, Reid AT, Knight DA, Bartlett NW, Park ...
JAMES W. GODING, in Monoclonal Antibodies (Third Edition), 1996 8.6.2 Fetal Calf Serum As a general rule, fetal calf serum (10–15%) must be added to the medium. The quality of fetal calf serum has greatly improved in recent years, and extensive testing of many different batches is now...