Serum free light chain assays for the detection of monoclonal protein. Study from a population of 135 hospitalized patientsMusset, L.Le Garff-Tavernier, M.IMMUNOANALYSE ET BIOLOGIE SPECIALISEE
In this study we demonstrated that fetal calf serum (FCS) depleted of IgG by protein G affinity chromatography (G-FCS) is superior to whole FCS or serum-free culture media as a culture supplement for the production of purified IgG monoclonal antibodies (MAb). One hundred ml FCS was applied...
However, administration of mAbs carries the risk of immune reactions such as acute anaphylaxis, serum sickness and the generation of antibodies. In addition, there are numerous adverse effects of mAbs that are related to their specific targets, including infections and cancer, autoimmune disease, and...
Serum M protein concentrations were significantly greater in patients with prolonged PT and were positively correlated with PT. ConclusionAn association between disease severity and prolonged PT is suggested by our finding that patients with multiple myeloma were more likely to have prolonged PT than ...
In conclusion, it appears from this study that semi-continuous cultivation in spinner flasks of hybridoma cells, after cloning and progressive adaptation, in a chemically defined, serum- and protein-free medium, permitted MAb secretion to be increased to a mean of 144 μg/ml, i.e., ...
The antibody response to a natural infection or an activeimmunization, however, is polyclonal. In other words, it involves many B cells, each of which recognizes a different antigenic determinant (epitope) of the immunizing antigen and secretes a different immunoglobulin. Thus the blood serum of ...
(Lane 1) and HUVEC treated with LPS and Brefeldin A (0.5 µg/mL of LPS for 24 h and 300 ng/mL of Brefeldin A for the last 20 h of LPS stimulation) (Lane 2) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then ...
Assessment of Oritavancin Serum Protein Binding across Species Biophysical methods to study the binding of oritavancin, a lipoglycopeptide, to serum protein are confounded by nonspecific drug adsorption to labware surf... FF Arhin,A Belley,G Mckay,... - 《Antimicrob Agents Chemother》 被引量: 31...
Finally, the experimental framework introduced in our study can be extended to other secreted proteins, such as antibodies from other species26or cytokines, because the modular design of our capture system (BG-agarose and SNAP-tag protein fusions) allows rapid adaptation. We hypothesize that light-...
Etesivumab (LY-CoV016, aka JS016, aka LY3832479) is a monoclonal antibody that targets the receptor-binding domain of the SARS-CoV-2 surface spike protein. REGEN-COV (formerly REGN-CoV-2 or REGEN-CoV-2) is a combination of two antibodies that bind to separate regions of the SARS-CoV...