Here, we report the cloning of theresistance genein this region using the method of sequencing trait-associated mutations (STAM).encodes a non-canonical resistance protein with a NAM domain and a ZnF-BED domain.
Plant height has a strong effect on crop productivity and an improved understanding of the genes underlying this trait will aid the selection of alternative dwarfing alleles in modern wheat germplasm. The approach we describe can be applied to rapidly identify candidate causative-induced mutations in ...
Low-coverage sequencing: implications for design of complex trait association studies. Genome Res. 2011;21:940–51. Article CAS PubMed PubMed Central Google Scholar Fumagalli M, Vieira FG, Korneliussen TS, Linderoth T, Huerta-Sanchez E, Albrechtsen A, Nielsen R. Quantifying population genetic ...
Mutations were detected using an adaption of the program used for SNP assay. The munged reads were competitively aligned by Maq, with default parameters, to the munged references. The maq pileup –v command was then used to generate text files encapsulating these alignments and associated base qu...
Low-coverage sequencing: implications for design of complex trait association studies Genome Res., 21 (2011), pp. 940-951 CrossrefView in ScopusGoogle Scholar Linderman et al., 2014 M.D. Linderman, T. Brandt, L. Edelmann, O. Jabado, Y. Kasai, R. Kornreich, M. Mahajan, H. Shah, A...
Sequencing trait-associated mutations (STAM) is a simple and straightforward gene cloning method that was developed in wheat. It uses full-length isoform sequencing (Iso-Seq) of the wild type as the reference and employs transcriptome sequencing of multiple, independently derived mutants for gene ...
Here, we report the cloning of the Pst resistance gene YrNAM in this region using the method of sequencing trait-associated mutations (STAM). YrNAM encodes a non-canonical resistance protein with a NAM domain and a ZnF-BED domain. We show that both domains are required for resistance. ...
CMS is a phenotypic trait that is widespread among plants and results in the inability of a plant to produce viable pollen [3], using CMS lines to obtain F1 hybrids, which is the preferred method in heterosis breeding. The CMS plants are usually obtained by natural populations [4,5], ...