The cap is thought to facilitate later processing steps, such... Schwer,Shuman - 《Rna-a Publication of the Rna Society》 被引量: 56发表: 1996年 Coupling pre-mRNA processing to transcription on the RNA factory assembly line. It has been well-documented that nuclear processing of primary ...
Secondary structures, i.e., the Watson–Crick andwobble base pairsproviding the scaffold of the three-dimensional structure, have turned out to be adequate to describe many aspects ofRNAmolecules in sufficient detail and accuracy for practical applications. This includes thermodynamic, kinetic, and evo...
Units of transcription and translation: sequence components of heterogeneous nuclear RNA and messenger RNA. Cell 4, 77-93.Lewin, B. (1975) Units of transcription and translation: sequence components of heterogeneous nuclear RNA and messenger RNA. Cell 4: pp. 77-93...
Michael S Bartlett, in Current Opinion in Microbiology, 2005 Here I discuss the role of DNA sequence in the formation of transcription initiation complexes, the transcription factors that decode the signals embedded in the DNA, and how these determinants govern RNAP function during initiation and gu...
MARS-seq/MARS-seq2.0FACSIn 96w/384w wellsRNase H and DNA pol INoBarcoded RT primersYesIn vitro transcription3' Microwell-seqNanowellsIn collection tubesTSOYesBarcoded RT primersYesPCR3' Quartz-seqFACSIn 96w/384w wellsPolyA tailing and primer ligationYes in principleLigation of barcoded Truseq...
Repetitive DNA sequences playing critical roles in driving evolution, inducing variation, and regulating gene expression. In this review, we summarized the definition, arrangement, and structural characteristics of repeats. Besides, we introduced diverse
transcription factor binding sites (TFBSs). However, the flanking sequences of cis-regulatory elements, have long been overlooked and often arbitrarily decided in promoter design. To address this limitation, we introduce DeepSEED, an AI-aided framework that efficiently designs synthetic promoters by ...
structure features using deep belief networks (DBNs). It includes the RNA structure information, obtained from another tool, as a count vector of kmers [21]. A disadvantage of Deepnet-rbp is that it requires complicated steps to estimate the binding preference [21]. Apart from CNN-based ...
The genomic DNA was the one extracted in the DNA sequencing experiment. To obtain the cDNA, the RNA extracted in the RNA sequencing experiment was subjected to reverse transcription reactions in a 20 μL reaction system containing 1 μg of total RNA, random primers, and 200 U of SuperScript...
MYC-MAX transcription factors bind to Enhancer boxes (a core element of the promoter that consists of six nucleotides) and activate transcription of the underlying genes [39]. The amino acid chain of MYC protein consists of 88 residues, ten of which are known DNA-binding sites indicating that...