Amplification method of any RNA target sequence, by transcription under the control of a promoter, in an RNA sample comprising said target sequence, which contacts said sample - with a reactive agent capable of hybridizing with said RNA comprising said target sequence, and - with an enzymatic ...
Learn about mRNA transcription. Discover where and how DNA is transcribed into RNA in prokaryotes and eukaryotes, and examine the final product of...
网络释义 1. 转录过程 ...辨认位点(recognition site) 二、转录过程(Process of transcription) 1. 原核生物的转录过程:转录的起始,转录空泡(transcript…www.docin.com|基于2个网页 例句 释义: 全部,转录过程 更多例句筛选 1. A polymerase that catalyzes the formation of RNA from a DNA template in the ...
Gene transcription is regulated by the chromatin landscape, including chromatin structure, DNA and histone modifications59,60. Accessible chromatin regions allow for the selective binding of regulatory elements, which is crucial to regulate gene expression in cell-type-specific or context-specific manners...
transcription process. For example, a group of nucleotides known as a promoter sequence lies near the beginning of most genes and provides a binding site for RNA polymerase to begin transcription. Similarly, other noncoding sequences near the promoter sequence function as protein binding sites that...
Rationally weighing the combination of the optimization parameters (e.g., codon adaptation, mRNA de novo synthesis and stability, transcription and translation efficiency) is important in order to achieve the most efficient expression of a given protein. Figure 1. Schematic representation of protein ex...
Fresh-cut corms of Chinese water chestnut (CWC) have become a very popular ready-to-eat fruit in Asian countries. However, the surface of fresh-cut CWC is prone to yellowing during storage, and the mechanism underlying this process remains unclear. In this study, RNA-Seq was used to ...
FED-BATCH IN VITRO TRANSCRIPTION PROCESS The present disclosure provides methods of in vitro transcribing a ribonucleic acid (RNA) of interest. In some embodiments, such methods include determinin... J Elich,AE Rabideau,M Shamashkin,... 被引量: 0发表: 2022年 ...
To characterize the atlas of porcine spermatogenesis, we profiled the transcriptomes of ~ 16,966 testicular cells from a 150-day-old pig testis through single-cell RNA-sequencing (scRNA-seq). The scRNA-seq analysis identified spermatogonia, spermatocytes, spermatids and three somatic cell types ...
Both the ribosomal DNA (rDNA) promoter and RNA polymerase I have to be “transcription ready,” and they must join together to form a functional initiation complex. The assembly of the intermediate and final complexes involved in this process requires a complex series of protein-DNA and protein...