那就需要我们做不同循环数的梯度PCR了，保证PCR过程中其他条件相同，只改变循环数（比如可以设计20.21.22.23.24.25.26等不同循环数），最后跑电泳，当条带明暗和粗细没有明显差异的那个循环数的前一个，即为达到平台期的循环数。我用的内参基因是UBQ7，之前实验经验得出它达到平台期的最佳循环数是20-24。为了...
对象及方法:采用半定量RT-PCR(semi-quantity RT-PCR,SQ-PCR)方法对服用伊马替尼慢性粒细胞白血病患者缓解或耐药前后的2个不同时点的9对(18份)标本进行了hOCT 1基因mRNA水平检测,并对SQ-PCR结果结合临床表现、实验室检查和细胞遗传学结果,分析其水平变化和与疾病缓解或伊马替尼耐药相关性。 3. Expression analysis...
Semi-quantitative reverse transcription and polymerase chain reaction (sqRT-PCR) is a simple and specific method for quantitative RNA in recent years. The relative quantity of a specific mRNA in the samples can be inferred by reverse transcription of mRNA into cDNA, and PCR amplification and ...
录聚峯酶连锁反应 (Semiquantitative RT-PCR) 分析其分化指标 ir.ym.edu.tw|基于5个网页 2. 半定量方法 用半定量方法 (semiquantitative RT-PCR) 来侦测正常子宫颈组织以及子宫颈癌组织其唾液酸转移ir.ym.edu.tw|基于1 个网页 3. 反转录聚合酶连锁反应 六、 反转录聚合酶连锁反应 (Semiquantitative RT...
A semi-quantitative PCR was developed and used to demonstrate the reverse transcriptase activity of the recombinant protein. 通过一种半定量PCR方法,检测到所表达的蛋白具有逆转录酶活性。 3. MethodsSemi-quantitative PCRmethod was used in this research to estimate PERV copy-number of four mini-pigs in...
2.A semi-quantitative PCR was developed and used to demonstrate the reverse transcriptase activity of the recombinant protein.通过一种半定量PCR方法,检测到所表达的蛋白具有逆转录酶活性。 3.Methods Semi-quantitative PCR method was used in this research to estimate PERV copy-number of four mini-pigs...
pcr reaction • Dilute cDNA (5 fold) and use between 1-5 microliters of cDNA per PCR reaction. • Add the following PCR cocktail per tube: 2.5 microliters 10X PCR buffer 1.5 microliters 25 mM MgCl2 1 microliter each primer (10pmol/microliter; 55° Tm) ...
5) Reverse real-time quantitative polymerase chain reaction 逆转录实时定量PCR6) Quantitative reverse transcription PCR 逆转录实时荧光定量PCR补充资料:逆转录 分子式:CAS号:性质:又称逆转录。以RNA为模板产生DNA分子的过程。 说明:补充资料仅用于学习参考,请勿用于其它任何用途。
Bone DNA was extracted and then assessed by the optimized semi-quantitative PCR and capillary electrophoresis of three products from the human mitochondrial DNA. The results show DNA survived for longer periods in samples stored in ambient conditions compared with underground burial conditions. There ...