那就需要我们做不同循环数的梯度PCR了，保证PCR过程中其他条件相同，只改变循环数（比如可以设计20.21.22.23.24.25.26等不同循环数），最后跑电泳，当条带明暗和粗细没有明显差异的那个循环数的前一个，即为达到平台期的循环数。我用的内参基因是UBQ7，之前实验经验得出它达到平台期的最佳循环数是20-24。为了...
Semiquantitative RT-PCR analysis of environmental influence on P450scc and PNMT mRNA expression in rat adrenal glands. Life Sci - Morita, Kuwanda, et al.Morita K, Kuwada A, Sano A, Sakata M, Sei H, Morita Y. Semi-quantitative RT-PCR analysis of environmental influence on P450scc and ...
0.2 microliters AMV RT 9 microliters Q • Incubate at 48° for 30 minutes, dilute 5 fold and store at -20°. pcr reaction • Dilute cDNA (5 fold) and use between 1-5 microliters of cDNA per PCR reaction. • Add the following PCR cocktail per tube: 2.5 microliters 10X PCR bu...
Semiquantitative RT-PCR analysis to assess the expression levels of multiple transcripts from the same sample. Biol Proced, 2001, 3(1): 19-25.Marone M,Mozzetti S,De Ritis D,et al.Semiquantitative RT-PCR analysis to assess the expression levels of multiple transcripts from the same sample....
录聚峯酶连锁反应 (Semiquantitative RT-PCR) 分析其分化指标 ir.ym.edu.tw|基于5个网页 2. 半定量方法 用半定量方法 (semiquantitative RT-PCR) 来侦测正常子宫颈组织以及子宫颈癌组织其唾液酸转移ir.ym.edu.tw|基于1 个网页 3. 反转录聚合酶连锁反应 六、 反转录聚合酶连锁反应 (Semiquantitative RT...
Semi-quantitativeRT-PCRanalysisshowedthat ZF3 wasexpressedinroot,stem,leaf,flower,immaturepodandembryoofchickpea. 半定量RT-PCR分析表明,ZF3在鹰嘴豆的根、茎、叶、花、幼荚和幼胚中均有表达。 www.boshuo.net 10. MethodsTheMALgeneexpressionin45cancerpatientswasdetectedwithRT-PCRandsemi-quantitative RT-...
1)Semi-quantitative analysis半定量分析 1.Semi-quantitative analysis of factors affecting gas hydrateformation conditions and its fractions;天然气水合物形成条件与含量影响因素的半定量分析 2.Based on the semi-quantitative analysis for all mineral medicine components in fluorites,the quantity assessment for...
semiquantitative双语例句1. The quantitative and semiquantitative results were consistent. 定量测定与定性的结果一致。 来自互联网 2. Semiquantitative RTPCR was used for verification of HDAC1 gene expression. 以半定量RT-PCR法检测瘤细胞中HDAC1基因表达加以验证。 来自互联网 3. For semiquantitative analysis...