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Eyes were then extensively flushed with sterilized PBS solution (~10 ml) using a syringe. At day 2, corneal neovascular outgrowths were imaged by a microscope. Mice were then randomly divided into 6 groups, treated only once with eye-drop instillation of either non-specific control IgG or ...
Before treatment, each rabbit was anesthetized using 3% sodium pentobarbital (1 mL/kg) through an injector placed in an ear vein. A 5 cm incision was made in the groin area, parallel to the left femoral artery’s course, situated between the abdomen and thigh. The femoral artery was ...
Then, triethylamine was added to the suspension by syringe. After that, 2-bromoisobutyryl bromide was added to the suspension dropwise under high stirring by syringe. A white precipitate of triethylamine salts, distinguishable from cellulose, started to appear, indicating the reaction of cellulose ...