Self-amplifying vectors can be introduced into target as RNA, DNA or .doi:10.1016/S0169-409X(97)00018-5JA WolffHans HerweijerAdv Drug Deliv RevHerwei jer H,Wolff J A. Self-amplifying vectors for gene delivery[J].Advanced Drug Delivery Reviews,1997.5-16.doi:10.1016/S0169-409X(97)00018-...
This chapter provides a brief introduction to nucleic acid-based vaccines and recent research in developing self-amplifying mRNA vaccines. These vaccines promise the flexibility of plasmid DNA vaccines with enhanced immunogenicity and safety. The key to realizing the full potential of these vaccines is...
Self-amplifying RNAs have shown enhanced antigen expression at lower doses compared to conventional mRNA, suggesting this technology may improve immunization. This review will explore how self-amplifying RNAs are emerging as important vaccine candidates for infectious diseases, the advantages of synthetic ...
Safety, tolerability and recommended phase 2 dose (RP2D) of an individualized, heterologous chimpanzee adenovirus (ChAd68) and self-amplifying mRNA (samRNA)-based neoantigen vaccine in combination with nivolumab and ipilimumab were assessed as primary endpoints in an ongoing phase 1/2 study in ...
RNA-based vaccines are an attractive candidate for this role: they are safe, are produced cell free, and can be rapidly generated in response to pathogen emergence. Two RNA vaccine platforms are available: synthetic mRNA molecules encoding only the antigen of interest and self-amplifying RNA (sa...
Self-Amplifying Replicon RNA Vaccine Delivery to Dendritic Cells by Synthetic Nanoparticles The review relates the DC endocytic pathways to immune response induction, and the potential advantages for these self-replicating RNA vaccines in the near ... K Mccullough,P Milona,L Thomann-Harwood,... -...
A DNA fragment encoding GFP under the control of the T7 promoter was prepared by amplifying the template plasmid pET-g5tag. We used PrimeSTAR HS (TaKaRa) and the primers 5′-GCGAAATTAATACGACTCACTATAGGG-3′ and 5′-GGTTATGCTAGTTATTGCTCAGCGG-3′ for amplification. Plasmids The amplified...
Due to low quantities of biomarker, amplifying release is necessary to gain appreciable responses. Nucleic acids have been used for both their biomarker-recognition properties and as stimuli, notably in amplified small-molecule release by nucleic-acid-templated catalysis (NATC). The multiple components...
We have developed an easily-manufactured, potent self- amplifying RNA (saRNA) vaccine against SARS-CoV-2 that is stable at room temperature. This saRNA vaccine is formulated with a nanostructured lipid carrier (NLC), providing stability, ease of manufacturing, and protection against degradation. In...
PCR is one of many examples of chemical processes that require precise temperature control of reaction mixtures with rapid and precise temperature changes between different stages of the process. PCR itself is a process for amplifying DNA, i.e., producing multiple copies of a DNA sequence from a...