Material science and engineering could construct new devices that may enable the enrichment of analytes and cells in vivo or ex vivo for subsequent testing.We functionalized two kinds of substrates with specific antibodies (IgG) that were directed against distinct cell surface antigens. One substrate...
Light-scatter gates were used to select the granulocyte region (neutrophils and eosinophils), excluding lymphocytes, monocytes, and basophils (46). Eosinophils (CD16-dim) and neutrophils (CD16-bright) were distinguished by their well-sep arated peaks on the CD16 (FITC-Leu1la) fluorescence ...
perfluoroheptanoic acid (PFHpA), perfluoropentanoic acid (PFPA), or mixtures thereof in concentrations ranging from 0.025 – 2.25 μM. Nanomolar and micromolar concentrations of PFAS were selected to maintain human relevance; however, it is important to note that in the context of endometrial...
There was little change in the numbers of cells staining for these antibodies through the three trimesters and at delivery with the exception of T lymphocytes bearing the LFA-1 antigen which were significantly less in the first trimester (P < 0.05). These data support the notion that T cell...
In contrast, chemokine CCL28, a ligand for CCR10 that is expressed primarily by IgA ASCs and some T lymphocytes, is expressed in mucosa of intestine and elsewhere [16]. In pigs, CCL25 recruits T cells and IgA ASCs that express CCR9 in the gut-associated lymphoid tissues and small ...
Recombination-activating genes (Rag1 and Rag2) are expressed in developing lymphocytes. Mice that are deficient for either of these genes fail to produce B or T cells owing to a developmental block in the gene rearrangement that is necessary for antigen receptor expression. Common variable immunode...
(3 µg/ml) for 18 h before they were cultured with the healthy individuals’ OCs and sAJ2 at a ratio of 1:2:4 (OCs:NK:sAJ2). On days 6, 9, 12, and 15 of co-culture, the numbers of lymphocytes were counted using microscopy (n = 70)(A). NK cells were treated and ...
With both kinds of substrates and three types of linker specific cells were enriched and collected out of samples that resemble in vivo conditions: CD4+ lymphocytes in blood samples and trophoblasts under flow conditions. Using~(l25)J-labelled IgG we demonstrated stable binding of antibodies to ...
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The RNA was shown to block the interaction of L-selectin with its natural ligands on high endothelial venules (HEV) in the Stamper-Woodruff assay at concentrations below 100 nM, and the ssDNA ligand blocked in vivo recruitment of lymphocytes dependent on L-selectin.; I have shown that ...