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While sbh1S3A/T5A cells accumulated significant amounts of GSTpGls1 in the cytosol (Fig. S4D), none of the proline-directed kinase mutants did (Fig. S4E). Our results suggest that despite their defect in Mns1ΔgpαF translocation, Δkns1,Δmck1, and cdc28-1 cells were competent ...
GST, glutathione transferase; LDH, lactate dehydrogenase; BSO, L-buthionine sulfoximine; CM, conditioned medium; TCA, tricarboxylic acid; PKC, protein kinase-C; ASK1, signal-regulating kinase 1; PTP1B, protein tyrosine phosphatase 1B; MPTP, mitochondrial permeability transition pore; MTT, 3-(4,...
表达氨基酸1-248aa BackgroundMay be involved in thymocyte signaling. ApplicationImmunology research Derived fromE.coli PurificationNI-NTA affinity purification FormulationLyophilized from a 0.2 μm filtered solution of Tris-HCl, PH8.0. ShippingThe product is shipped at -20°C temperature. Upon receipt,...
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Sso2 also co-immunoprecipitated with Sec3 from cell lysates, suggesting their interaction in cells (Fig. 1b). Recombinant Sec3N bound to both Sso2 (Fig. 1c) and Sso1, a close paralog of Sso2 in yeast (Supplementary Fig. 1b). As a negative control, glutathione S-transferase (GST)-...
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接着,他们做了一个肽竞争实验。肽1模拟紧密连接蛋白-1的尾部,但肽2没有C末端YV/AA突变,这阻碍了Sec24C与GST-紧密连接蛋白-1尾部的相互作用,进一步证明了YV对于作用的必要性(图6.c)。 综上,该工作完善了对紧密连接蛋白在细胞表面...
The present invention discloses Men protein, GST2, Rab-RP1, Csp, F-box protein Lilina/FBL7, ABC50, coronin, Sec61 alpha, or VhaPPA1-1 and homologous proteins regulating the energy homeostasis and the metabolism of triglycerides, and polynucleotides, which identify and encode the proteins ...
pCMV-SPORT6-SEC14L4质粒结构是一个大肠杆菌表达载体,Tac强启动子可以驱动GST促溶标签和目的基因融合表达,LacI阻碍蛋白和Laco操作子使本质粒在没有加入IPTG之前禁止表达,防止其影响细菌生长。表达后的融合蛋白可用凝血酶蛋白酶切割,再过一次GST柱子即可清除掉GST标签。 产品相册pCMV-SPORT6-SEC14L4质粒结构TTTCCTGCGTT...