and even the best-run laboratories can experience equipment failure. An established cell line is a valuable resource, and its replacement is expensive and time consuming. Therefore, it is vitally important that they are frozen down and preserved for long-term storage. A properly maintained frozen ...
A Flp recombinase expression plasmid, pOG44, for expression of the Flp recombinase under the control of the human CMV promoter A control expression plasmid containing the chloramphenicol acetyl transferase (CAT) gene, which when co...
Moreover, several genes involved in polyamines and carbohydrates metabolisms resulted downregulated by AMF inoculum, in agreement to the GO term “carbohydrate metabolic process”, significantly enriched in the downregulated genes set. Polyamines are important regulators of cellular ROS homeostasis55, while...
Use of patellar tendon autograft for anterior cruciate ligament reconstruction in the rabbit: a long-term histologic and biomechanical study. J Orthop Res. 1989;7:474–85. Article CAS PubMed Google Scholar Shino K, Kawasaki T, Hirose H, Gotoh I, Inoue M, Ono K. Replacement of the ...
After 120 s of adaptation, the animals remained there for 240 s during which the total time of freezing responses was assessed. The rats were not exposed to CS or US this day. As on the previous day, the cage was cleaned with isopropyl alcohol between trials. Cued fear conditioning was ...
There is still a long way to go for humans to produce food in space for long-term living. The most important thing is to ensure that the crops grown in space can be safely eaten. If the food tastes great, then all the more better....
recognized the need for a systematic, long-term collection of brain tissue and accompanying behavioral data from dogs, which represent different stages of aging and CCD progression, as it was impossible to obtain an appropriate sample size within a standard project time-frame to search for associati...
Whether this is also true for cells subjected to long-term confinement remains unclear at present. To investigate this, photopatterning and microfluidics are employed to fabricate a high-throughput device that circumvents limitations of previous cell confinement models and enables prolonged culture of ...
as ina new paradigm for understanding diabetes.This usage was acceptable to 91 percent of the Usage Panel in our 2009 survey. Applications of the term in other contexts show that it can sometimes be used more loosely to mean "the prevailing view of things." The Usage Panel also accepts thes...
A statistically compiled test battery for feasible evaluation of knee function after rupture of the Anterior Cruciate Ligament–derived from long-term follow-up data PLoS One Schelin, L., Tengman, E., Ryden, P., & Häger, C. Umeå University 2017 Using facial expressions of emotion as a...