Synovial mesenchymal stem cells (MSCs) have high freeze–thaw tolerance, whereas human umbilical vein endothelial cells (HUVECs) have low freezing tolerance. The differences in cell type-specific freeze–thaw tolerance and the mechanisms involved are unclear. This study thus aimed to identify the bio...
Single-cell suspensions were then viably frozen in fetal bovine serum (FBS) supplemented with 10% DMSO (Sigma-Aldrich) using a controlled freezing unit (Cool Cell LX). Cryopreserved tumor dissociates (TD) and healthy PBMCs were resuscitated for Mass Cytometry analyses by rapid thawing and slow...
4B). We stimulated endocytic uptake of EGFR by adding EGF ligand for defined periods of time before high-pressure freezing, freeze substitution and electron microscopy (EM). We decided to count ILVs between 40 and 60 nm diameter, since ESCRT-dependent ILVs in human cells were found to be...
slow freezingultrastructurecryodamageIn this case-control study, we investigated the potential associations of MMP-2 and MMP-9 gene promoter region polymorphisms as well as MMP-2 promoter haplotypes with susceptibility to endometriosis in women of caucasian origin. The results demonstrated that ...
the country, the sight of my breath on the freezing air makes me smile. i may whine about the below zero temperatures when i return to the midwest in january and joke that i don’t understand how anyone lives in this climate, but beneath my complaints, i am reveling in the sensations...
Minced tissues were thawed, centrifuged to remove the freezing medium, and enzymatically dissociated using epithelial cell culture medium supplemented with trypsin-EDTA (Invitrogen, Carlsbad, CA, USA) and 2.5 mg/mL of dispase (Roche, Indianapolis, IN, USA). 2.2. Flow Cytometry and Cell Sorting A...