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Briefly, PFA-fixed samples were washed in phosphate-buffered saline (PBS), cryo-preserved in 30% sucrose and cut into 80 µm thick sections on a sliding freezing microtome (LeicaSM2010R). After overnight ultraviolet (UV) irradiation for autofluorescence reduction the sections were permeabilized ...
the country, the sight of my breath on the freezing air makes me smile. i may whine about the below zero temperatures when i return to the midwest in january and joke that i don’t understand how anyone lives in this climate, but beneath my complaints, i am reveling in the sensations...
Plants from temperate regions are able to withstand freezing temperatures and to increase their freezing tolerance during exposure to low, but non-freezing... C Gery,E Zuther,E Schulz,... - 《Plant Science An International Journal of Experimental Plant Biology》 被引量: 62发表: 2011年 1 Short...
Single-cell suspensions were then viably frozen in fetal bovine serum (FBS) supplemented with 10% DMSO (Sigma-Aldrich) using a controlled freezing unit (Cool Cell LX). Cryopreserved tumor dissociates (TD) and healthy PBMCs were resuscitated for Mass Cytometry analyses by rapid thawing and slow...
Samples were then rinsed in PBS and embedded in cryo-molds with Optimal Cutting Temperature (OCT) before freezing. 40-60 μm (for 3D imaging, LGR5 staining) or 10 μm (for 2D imaging, p16 staining) thick sections of fixed skin were cryosection onto poly L-lysine coated coverslips or...
4B). We stimulated endocytic uptake of EGFR by adding EGF ligand for defined periods of time before high-pressure freezing, freeze substitution and electron microscopy (EM). We decided to count ILVs between 40 and 60 nm diameter, since ESCRT-dependent ILVs in human cells were found to be...
Human-like behavioral variability blurs the distinction between a human and a machine in a nonverbal Turing test 2022, Science Robotics Belief in Sharing the Same Phenomenological Experience Increases the Likelihood of Adopting the Intentional Stance Toward a Humanoid Robot 2022, Technology, Mind, and...
Alternatively, bones from young mice and also other tissues were collected by snap freezing. Paraffin sections (5 μm thick) were deparaffined/hydrated, submitted to different antigen retrieval (AR) methods, and, when necessary, quenched for endogenous peroxidase (with 2% H2O2 in PBS). ...
However, Double mice showed a significantly lower percent time freezing during the context- but not cue-dependent fear conditioning assays (Figure 1M, right panel). The lack of significant difference in Res and Double mice during cue-dependent fear conditioning is possibly the result of leaky UBC...