Regulation of the cell cycle is dependent on protein degradation by the ubiquitin–proteasome system. Two major ubiquitin ligases, the anaphase-promoting complex or cyclosome (APC/C) and SCF complex, are responsible for the periodic proteolysis of many regulators of the cell cycle. The receptor ...
D-type cyclins are important cell cycle regulators that promote cellular proliferation in response to growth factors by inactivation of the retinoblastoma protein (Rb). Cyclin D1 has been shown to be overexpressed in several cancer types and to act as an oncogene in breast cancers. As D-type ...
ANAPHASE-PROMOTING COMPLEX/CYCLOSOME (APC/C). Anaphase is the phase of mitosis during which condensed chromosomes separate into sister chromatids and move along the mitotic spindles to opposite poles of the cell. The APC/C is a multi-subunit E3 ubiquitin ligase with at least two alternative form...
Cul1 is a core component of the evolutionarily conserved SCF-type ubiquitin ligases that target specific proteins for destruction. SCF action contributes to cell cycle progression but few of the key targets of its action have been identified. We found th
There are over 600 different ubiquitin ligase in the human genome. Among them, the Skp1-Cullin-F-Box (SCF) complexes and the Anaphase Promoting Complex/Cyclosome (APC/C) are best known for their roles in cell-cycle control7. For SCF complexes, the F-box protein determines substrate specific...
Cyclin D1 and D3 associate with the SCF complex and are coordinately elevated in breast cancer 来自 EBSCO 喜欢 0 阅读量: 32 作者:A Russell,MA Thompson,J Hendley,L Trute,J Armes,D Germain 摘要: D-type cyclins are important cell cycle regulators that promote cellular proliferation in ...
ofaSkp1-Cul1-F-boxcomplex.Significantly,JFKinhibitsp53- dependenttranscription,anddepletionofJFKstabilizesp53,pro- motescellapoptosis,arrestscellsintheG 1phase,andsensitizes cellstoionizingradiation-inducedcelldeath.Thesedataindicate thatJFKisacriticalnegativeregulatorofp53andrepresentsa ...
因此,本实验室对病原微生物刺激后的小鼠原代腹腔巨噬细胞及单核巨噬 细胞细胞系RAW264.7细胞进行了E3泛素连接酶表达谱芯片的分析,从该芯片结 果中,我们关注到了一群以往在天然免疫中功能鲜有报道的E3泛素连接酶家族 SCF复合体(SKPl-CULl-F—BOXprotein complex),该复合体的标志性蛋白为发 挥底物蛋白识别功能的F...
degradation. Disruption of ING3 degradation stimulated ING3-induced G1 cell-cycle arrest and enhanced ultraviolet-induced apoptosis. Taken together, our data show that ING3 is degraded by the ubiquitin-proteasome pathway through the SCF(Skp2) complex and interruption of ING3 degradation enhances the...
with all of the core members of the tRNA ligase complex (Fig.4eand Supplementary Table5), which is subject to oxidative hydroxyl radical damage, in particular in the presence of Cu(ii)32. MS analysis of recombinantly expressed and purified tRNA ligase complex confirmed CTAP formation under oxi...