SARS-CoV-2 possesses a ~30-kb positive-sense RNA genome encoding ~29 proteins including four structural proteins: spike (S), nucleocapsid (N), matrix (M), and envelope (E)7,8. Homotrimers of the S glycoprotein coat the SARS-CoV-2 virion and engage the viral receptor, angiotensin conver...
Adding to these findings, our results demonstrate that fat is a common extrapulmonary site where SARS-CoV-2 RNA can be detected in patients who died of COVID-19. SARS-CoV-2 spike protein was found across the adipose tissue of subjects who had detectable viral genome in fat (Supplementary ...
The human anti-SARS-CoV-2 Spike RBD antibody (IgG and IgM isotypes) was spiked into a negative serum sample respectively, and then tested by QuantiVirus™ Anti-SARS-CoV-2 IgG Test. A strong binding was observed for IgG isotype while no binding interaction was observed between the anti-...
There is no standard reference SARS-CoV-2 antigen material available; accordingly, absolute analytical sensitivity cannot be calculated, however, all examined specimens with negative screen results using RBD protein also tested negative against spike protein at 1:80 dilution. Cross-reactivity of non-SAR...
Non-targeted whole genome sequencing is a powerful tool to comprehensively identify constituents of microbial communities in a sample. There is no need to direct the analysis to any identification before sequencing which can decrease the introduction of
Non-linear regression analysis of each dilution series for noswab, SYN, CGp, and TMI swabs Full size image Hospital proof of concept study Based on the results from these initial experiments, we conducted a proof-of-concept study in the clinical setting by performing RT-qPCR for the SARS-...
Figure 1. Concentration of IgG anti-SARS-CoV-2 spike receptor-binding domain (RBD) titers (logarithmic scale 10) reported in the box-whisker plots (and outliers) for individuals with previous infection at M1 and vaccinated individuals without previous infection for the three different moments of ...
Results for B.1.617.2 were similar with the 478K probe (B) used in singleplex (dotted curves) or triplex reactions (solid curves). Nonspecific signals were not detected in any channel. Table 1. Spike SNP assay primers and probes used in the current study. 3.2. SNP Detection in ...
SARS-CoV-2 and its Spike protein directly stimulated platelets to facilitate the release of coagulation factors, the secretion of inflammatory factors, and the formation of leukocyte–platelet aggregates. Recombinant human ACE2 protein and anti-Spike monoclonal antibody could inhibit SARS-CoV-2 Spike ...
with a 1.6 °C/s ramp up and ramp down rate. The limit of detection (LoD) of the assay was performed by serial dilution of γ-irradiated SARS-CoV-2 (0–5.0 × 105viral copies/mL) used to spike pooled fresh saliva samples. LoD experiments were repeatedly performed at least five...