31 Second, the study used SARS-CoV-2 antibodies to define infection and did not have access to verification via PCR. Nevertheless, the antibody measurements and strategy were previously shown to be highly specific and sensitive.18,32 Third, although the sampling was relatively frequent, it was ...
Additionally, PCR tests for SARS-CoV-2 during the first wave in Germany were mostly limited to the household index case, meaning it is possible that infected individuals were not identified as such, despite the multi-assay serological approach. However, the in-depth characterization of the ...
Of note, two participants who had been diagnosed with SARS-CoV-2 infection based on PCR results had negative results for SARS-CoV-2 antibodies. The numeric values of the cutoff index (COI) for the SARS-CoV-2 antibody were divided into a group showing high values (≥ 10) and a group...
Over the last few years, we’ve all been given a valuable lesson in both the promise and limitations of advanced molecular biology methods for clinical diagnostics. Polymerase chain reaction (PCR) was held up as the “gold standard” of COVID-19 testing, but the cost, complexity, and need...
SARS-CoV-2 RNA in cell culture supernatant was detected by RT-PCR as described previously.11 SARS-CoV-2 sgRNA in homogenized tissue samples was quantified by RT-PCR as published previously12 using SARS-CoV-2 (isolate HH-113)–infected Vero cells (CRL-1586; ATCC) as a control. Cell ...
re-suspended in RNase-free water and precipitated again with 2 M LiCl overnight at − 20 °C. Amplification and detection were performed using the iCycler IQ Real-time PCR detection system with Luna® universal One-Step RT-qPCR kit. Quantitative real-time PCR was used to measure the ...
Initially, we used an iSeq instrument to sequence a PCR-amplified region of the SARS-CoV-2 spike protein gene. This region spanned spike protein amino acid residues 434–505, which includes the receptor binding domain (RBD) (Fig. 1A). Beginning in April 2021, we switched to using a Mi...
SARS-CoV-2检测和结果从国家新冠肺炎数据库中检索,该数据库包括自大流行开始以来在卡塔尔进行的所有PCR检测结果。20,21,23在这些日期之间检测呈阳性的人群中,我们确定了在阳性样本上进行变异基因分型的人群。对于每一个确诊为δ变异感染...
cross-reactivity test with other tested viruses indicated that the SARS-CoV-2 CRISPR/Cas12a detection system had great specificity (Fig.1C and Supplementary Fig. S1D). The results of CRISPR/Cas12a assay were in 100% agreement with qRT-PCR, meaning it correctly identified and differentiated all...
we reported a SARS-CoV-2 replicon system with PCR amplicon-based strategy. The advantage of this system is its technical simplicity. Additionally, this system enabled us to produce a replicon without generating genetically modifiedE. coli. Thus, bacteriotoxic elements in the SARS-CoV-2 genome do...