Viral RNA copies were quantified in apical supernatants by qRT-PCR (n = 1 experiment). Full size image Epithelial interferon production provides another key defense mechanism, that can occur in the absence of immune cell infiltration, and thus represents one of the earliest antiviral response ...
we performed mass spectrometry analysis using human HEK 293T cells that ectopically express mCherry and FLAG-tagged Nsp6. A total of 661 proteins were uniquely detected in the Nsp6 expressing cells, i.e., proteins without any detectable pulldown in the control cells...
In total, 48 samples were analyzed by qRT-PCR. Result: We had 16 participants, 47% were females and 53% were males; 2/16 [12.5%] patients showed the presence of SARS-CoV-2 in tears and conjunctival swabs. Both the infected patients were females, and they di...
Notably, HA-reactive memory T cells were detected at normal levels in patients with SLE (Extended Data Fig. 8b), although the AIM+ HA CD8+ pool still failed to generate normal TEM cells (Extended Data Fig. 8c), suggesting that CD8+ T cell defects might be mediated by associated lupus-...
The standard molecular method for COVID-19 diagnosis is real-time reverse transcription polymerase chain reaction (rRT-PCR) in which RNA from SARS-CoV-2 is detected. This technique is based on the amplification of complementary DNA (cDNA), synthesized from a conserved region of the viral genome...
For most donors HLA genotyping was performed using the One Lambda ALLType kit, which uses multiplex PCR to amplify full HLA-A/B/C gene sequences, and from exon 2 to the 3′ UTR of the HLA-DRB1/3/4/5/DQB1 genes. Prepared libraries were run on an Illumina MiSeq sequencer using stand...
timequantitativePCRassays. Results:LHsignificantlyinhibitedSARS-CoV-2replicationinVeroE6cellsandmarkedlyreducedpro-in- flammatorycytokines(TNF-α,IL-6,CCL-2/MCP-1andCXCL-10/IP-10)productionatthemRNAlevels. Furthermore,LHtreatmentresultedinabnormalparticlemorphologyofvirionincells. ...
SARS-CoV-2 infection of human testicular tissue and abnormal postinfection levels of sex hormones have been observed [4,6]. Low T levels were found to be an indicator of the most severe clinical outcomes for male COVID-19 patients [6]. However, whether low T was present prior to infectio...
To date, no replication-competent virus has been detected in breast milk, yet the presence of SARS-CoV-2 RNA has been documented through reverse transcription polymerase chain reaction (RT-PCR)-based assays [61]. Conversely, breast milk has been documented to contain SARS-CoV-2-specific IgG,...
Patients have also been reported with liver injury as detected by the presence of elevated liver enzymes, including abnormal levels of alanine and aspartate aminotransferases (ALTandAST), elevated levels of gamma-glutamyl transferase (GGT), and mild elevations in serum bilirubin. Although most of th...