SARS-CoV-2CALMODULINRECEPTOR antibodiesANGIOTENSIN converting enzymeOmicron SARS-CoV-2 is rapidly spreading worldwide. To delineate the impact of emerging mutations on spike's properties, we performed systematic
heptad repeat 1; HR2, heptad repeat 2; IC, intracellular domain; NTD, N-terminal domain; SD1, subdomain 1; SD2, subdomain 2; TM, transmembrane region.b, Sequence and secondary structures of SARS-CoV-2 RBD. The RBM sequence is shown in red.c, Overall structure of the SARS-CoV-2 RB...
(2020). (A) Schematic of spike structure of SARS-CoV-2 colored by domain and side views of spike of SARS-CoV-2. SS: signal sequence, S2′: S2′ protease cleavage site, FP: fusion peptide, HR1: heptad repeat 1, CH: central helix, CD: connector domain, HR2: heptad repeat 2, TM...
Reconstructive 3D mapping of the SARS-CoV-2 spike in complex with Fab 71281-33 indicated that the epitope of that antibody is near the receptor-binding motif (RBM) of the RBD. Notably, the nsEM reconstruction showed Fab 71281-33 binding to the RBD in both the up and down states (...
作者先比较公开的S蛋白结构和序列,统计分析后发现,RBM是S蛋白中最不保守的区域,且具有一定程度的适应突变的结构可塑性,如SARS-CoV以及其他动物CoV的RBM与SARS-CoV-2不同,但都能结合ACE2。然后作者发现了在2020年10月有两株比较流行的RBM变体N439K,最早在3月份发现,属于B.1系,具有D614G背景。结构和SPR实验显示...
The spike protein (S) of SARS-CoV-2 is responsible for viral attachment and entry, thus a major factor for host susceptibility, tissue tropism, virulence and pathogenicity. The S is divided with S1 and S2 region, and the S1 contains the receptor-binding domain (RBD), while the S2 contain...
The major domains of the S2 subunit are two heptad repeats (HR1 and HR2), a central helix (CH), fusion peptide (FP) and a connector domain (CD) which connects the spike to the virus membrane. The SARS-CoV-2 S protein interacts with the human angiotensin-converting enzyme ...
CoV-1 [45]. Although RaTG13 is 96% similar to SARS-CoV-2, but the receptor binding domain (RBD) of SARS-CoV2 spike protein shares only 85% similarity with the RaTG13 and only one out of six critical amino acid residues of RBD is similar in RaTG13 and SARS-CoV-2 [46,47,48]...
SARS-CoV-2 RBD utilizes its external subdomain to recognize the N-terminal peptidase domain of ACE2, similar to that reported for SARS-CoV.25This external subdomain of RBD was named as the receptor-binding motif (RBM), and its two-stranded β-sheet component, also called β-hairpin, forms...
SARS-CoV-2 spike protein S binds to ACE2 through the receptor-binding domain (RBD) and is proteolytically activated by the human protease TMPRSS2, which loosens the structural constraints on the fusion peptide (FP) and initiates a cascade of refolding events (e.g., formation of the three-...